A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity
A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity
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DOI：
10.1038/ng2056
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发表时间：
2007-07-01
期刊：
影响因子：
30.8
通讯作者：
Vandenbussche, Michiel
中科院分区：
文献类型：
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作者：
Cartolano, Maria;Castillo, Rosa;Vandenbussche, Michiel
人们普遍认为植物 microRNA (miRNA) 及其靶标 (1,2) 的深层系统发育保守性表明其调控功能保守。我们发现矮牵牛（3）的盲（bl）突变体和金鱼草（4,5）的瘘（fis）突变体具有相似的同源异型表型，是两个同源miRNA编码基因的隐性等位基因。 BL 和 FIS 基因控制同源异型 C 类基因 (6,7) 对内花轮的空间限制，但它们普遍存在的早期花表达模式与 C 基因表达模式的潜在作用相矛盾。我们为花朵中心的 MIRFIS 和 MIRBL 基因的意外功能提供了遗传证据，并提出了它们调节作用的动态机制。值得注意的是，拟南芥（Arabidopsis thaliana）是一个关系较远的物种，也含有这种 miRNA 模块，但似乎没有使用它来将早期 C 基因表达限制在花的中心。
It is commonly thought that deep phylogenetic conservation of plant microRNAs ( miRNAs) and their targets(1,2) indicates conserved regulatory functions. We show that the blind ( bl) mutant of Petunia hybrida(3) and the fistulata ( fis) mutant of Antirrhinum majus(4,5), which have similar homeotic phenotypes, are recessive alleles of two homologous miRNA- encoding genes. The BL and FIS genes control the spatial restriction of homeotic class C genes(6,7) to the inner floral whorls, but their ubiquitous early floral expression patterns are in contradiction with a potential role in patterning C gene expression. We provide genetic evidence for the unexpected function of the MIRFIS and MIRBL genes in the center of the flower and propose a dynamic mechanism underlying their regulatory role. Notably, Arabidopsis thaliana, a more distantly related species, also contains this miRNA module but does not seem to use it to confine early C gene expression to the center of the flower.