Improved Fat Graft Survival by Different Volume Fractions of Platelet-Rich Plasma and Adipose-Derived Stem Cells
Improved Fat Graft Survival by Different Volume Fractions of Platelet-Rich Plasma and Adipose-Derived Stem Cells
复制标题
通过不同体积分数的富含血小板的血浆和脂肪干细胞提高脂肪移植物的存活率。
DOI：
10.1093/asj/sju046
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发表时间：
2015-03-01
影响因子：
2.9
通讯作者：
Tian, Weidong
中科院分区：
文献类型：
--
作者：
Li, Feng;Guo, Weihua;Tian, Weidong
背景：软组织填充术的成功被移植脂肪组织的低存活率所抵消。目的：研究不同体积分数富血小板血浆（PRP）与脂肪干细胞（ASCs）联合应用对脂肪移植的远期效果。采用细胞计数试剂盒-8和实时聚合酶链反应（PCR）分别评价培养基中PRP（0%、10%、20%和30%;体积/体积[v/v]）对ASC增殖和成脂分化的影响。将一种由颗粒脂肪、PRP和ASCs组成的新型脂肪注射液皮下移植到裸鼠体内。移植后10、30、60和90天对移植物进行体积和组织学评价。在PRP诱导的ASCs中，成脂相关基因、过氧化物酶体增殖物激活受体-γ、脂蛋白脂酶和亲脂蛋白的表达上调。与其他组相比，20%（v/v）和30%（v/v）PRP形成的颗粒脂肪移植物显著改善残余体积。在30、60和90天时，在20%（v/v）和30%（v/v）PRP组中观察到更多完整的脂肪细胞和毛细血管形成，但空泡化较少。然而，20%（v/v）和30%（v/v）PRP组之间没有显着差异，观察保留脂肪移植物和改善histologic.Conclusions：脂肪移植与20%（v/v）PRP和ASCs构成一个适当的移植策略，提高移植物的存活率，并提供了一个潜在的方法，在整形和重建外科软组织修复。
Background: The success of soft-tissue augmentation is offset by the low survival rates of grafted fat tissue. Research shows that adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) are beneficial to tissue healing.Objectives: To evaluate the long-term effects of different volume fractions of PRP combined with ASCs on fat graft.Methods: ASCs were isolated from human fat tissue, and PRP was obtained from human blood. Cell count kit-8 and real-time polymerase chain reaction (PCR) were used to evaluate the influence of PRP (0%, 10%, 20%, and 30%; volume/volume [v/v]) in medium on ASC proliferation and adipogenic differentiation, respectively. A novel lipoinjection consisting of granular fat, PRP, and ASCs was subcutaneously transplanted into nude mice. The grafts were volumetrically and histologically evaluated 10, 30, 60, and 90 days after transplantation.Results: The addition of PRP improved ASC proliferation. Expression of adipogenic-related genes, peroxisome proliferator-activated receptor-gamma, lipoprotein lipase, and adipophilin were up-regulated in PRP-induced ASCs. Compared with other groups, granular fat grafts formed with 20% (v/v) and 30% (v/v) PRP significantly improved residual volumes. More intact adipocytes and capillary formation, but less vacuolization, were observed in the 20% (v/v) and 30% (v/v) PRP groups at 30, 60, and 90 days. However, no significant difference was observed between the 20% (v/v) and 30% (v/v) PRP groups in retaining fat grafts and improving histology.Conclusions: Fat grafting with 20% (v/v) PRP and ASCs constitutes an appropriate transplantation strategy for improving graft survival and provides a potential approach for soft-tissue restoration in plastic and reconstructive surgery.