The permeability of the human erythrocyte to sodium and potassium.
The permeability of the human erythrocyte to sodium and potassium.
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DOI：
10.1085/jgp.36.1.57
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发表时间：
1952-05
期刊：
影响因子：
--
通讯作者：
SOLOMON AK
中科院分区：
文献类型：
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作者：
SOLOMON AK
用放射性示踪剂测定了人红细胞在体外对Na和K的渗透性，观察了系统的稳态。平均向内K通量为1.67毫当量。/l电池小时，表观活化能为12300±1300卡/摩尔。在研究的浓度范围内（4至16毫当量），向内的钾通量与外部的钾浓度无关。K /升等离子体)。Rb似乎与K竞争进入细胞，而Na和Li则没有。平均向内Na通量为3.08±0.57 m.eq。钠/升细胞小时，向内运输表观活化能为20,200±2700卡路里/mol，向外运输表观活化能为14,900±3,400卡路里/mol。向内的钠通量依赖于外部的钠浓度，但不是线性的。Li似乎与Na竞争向内运输，而K和Rb则没有。一个近似的最大估计表明，阳离子运输所需的能量仅为8.8卡路里/mol升细胞小时，而葡萄糖消耗的能量为110卡路里/mol升细胞小时。提出了Na和K输运的一个工作假设。
Measurements have been made on the permeability of the human erythrocyte to Na and K in vitro, using radioactive tracers to observe the system in the steady state. The average inward K flux is 1.67 m.eq./liter cells hour, and the apparent activation energy is 12,300 ± 1300 calories/mol. The inward K flux is independent of the external K concentration in the range of concentrations studied (4 to 16 m.eq. K/liter plasma). Rb appears to compete with K for transport into the cell, whereas Na and Li do not. The average inward Na flux is 3.08 ± 0.57 m.eq. Na/liter cells hour, and the apparent activation energies are 20,200 ± 2700 calories/mol for inward transport, and 14,900 ± 3,400 calories/mol for outward transport. The inward Na flux is dependent on the external Na concentration, but not in a linear fashion. Li appears to compete with Na for inward transport, whereas K and Rb do not. An approximate maximum estimate shows that the energy required for cation transport is only 8.8 calories/mol liter cells hour of the 110 calories/mol liter cells hour available from the consumption of glucose. A working hypothesis for the transport of Na and K is presented.