Up-regulation of MUC5AC and MUC5B mucin genes in chronic rhinosinusitis
Up-regulation of MUC5AC and MUC5B mucin genes in chronic rhinosinusitis
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DOI：
10.1001/archotol.130.6.747
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发表时间：
2004-06-01
影响因子：
--
通讯作者：
Lee, HM
中科院分区：
文献类型：
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作者：
Kim, DH;Chu, HS;Lee, HM
背景：粘液产生过多和分泌过多是上呼吸道疾病的特征。导致慢性鼻窦炎粘液分泌过多的主要机制尚不清楚。粘液分泌过多常伴有杯状细胞和粘膜下腺细胞增生。确定哪些粘蛋白基因信使rna （mrna）在窦粘膜中表达是很重要的。目的：研究人鼻窦黏膜MUC5AC和MUC5B mrna的表达及其定位，比较正常和慢性鼻窦黏膜MUC5AC和MUC5B mrna的表达。设计：慢性上颌鼻窦炎粘膜标本20份，正常上颌窦黏膜标本20份；从鼻窦中提取rna。MUC5AC和MUC5B进行半定量逆转录聚合酶链反应。这些蛋白的定位是通过免疫组织化学分析来寻找的。结果：慢性鼻窦炎组织MUC5AC和MUC5B mRNA表达水平明显高于正常鼻窦炎组织（P = 0.02）。在炎症的窦黏膜中，MUC5AC蛋白在表面上皮杯状细胞的细胞质中表达，MUC5B蛋白仅在粘膜下腺的粘液细胞和窦黏膜上皮中表达。而在正常鼻窦黏膜中，MUC5AC和MUC5B蛋白分别在鼻窦上皮和粘膜下腺中低水平表达。结论：提示慢性鼻窦炎呼吸道分泌的主要成分MUC5AC和MUC5B的上调可能在慢性鼻窦炎窦性高分泌的发病机制中起重要作用。
Background: Excess mucus production and hypersecretion characterize upper airway diseases. The primary mechanisms leading to mucus hypersecretion in chronic rhinosinus inflammation are not well understood. Mucus hypersecretion is commonly accompanied by goblet cell and submucosal gland cell hyperplasia. It is important to identify which mucin gene messenger RNAs (mRNAs) are expressed in the sinus mucosa.Objectives: To investigate the expression of MUC5AC and MUC5B mRNAs and localization of these proteins in human sinus mucosa and to compare the expression of MUC5AC and MUC5B mRNAs in normal and in chronic sinus mucosa.Design: Twenty chronic maxillary sinusitis mucosa samples and 20 normal maxillary sinus mucosa samples were obtained; RNAs were extracted from sinus. mucosa, and semiquantitative reverse transcription-polymerase chain reaction was performed for MUC5AC and MUC5B. Localization of these proteins was sought by using immimohistochemical analysis.Results: The levels of MUC5AC and MUC5B mRNA in chronic rhinosinusitis were significantly increased compared with those in normal sinus mucosa (P = .02). In inflammed sinus mucosa, MUC5AC protein was expressed in the cytoplasm of the goblet cell in the surface epithelium, and MUC5B expression was restricted to mucous cells of the submucosal glands and to the epithelium of sinus mucosa. However, in the normal sinus mucosa, MUC5AC and MUC5B proteins were expressed at low levels in the sinus epithelium and submucosal glands, respectively.Conclusion: These results suggest that up-regulation of MUC5AC and MUC5B, which are major components of respiratory secretion in chronic rhinosinusitis, may play important roles in the pathogenesis of sinus hypersecretion in chronic rhinosinusitis.