SOD3 and eNOS genotypes are associated with SOD activity and NO(x).
SOD3 and eNOS genotypes are associated with SOD activity and NO(x).
复制标题
SOD3 和 eNOS 基因型与 SOD 活性和 NOx 相关
DOI：
10.3892/etm.2014.1720
复制
发表时间：
2014-07
影响因子：
2.7
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Dong X;Li D;Liu H;Zhao Y
氧化应激在高血压的发病机制中起着重要作用，其特征是活性氧生成增加和/或抗氧化酶活性降低。寻找与高血压氧化应激状态相对应的分子标志物有助于高血压的抗氧化治疗。本研究分析了超氧化物歧化酶(SOD)和内皮型一氧化氮合酶(ENOS)作为高血压氧化应激反应的标志物。测定高血压患者和正常血压对照组血浆超氧化物歧化酶(SOD)活性和一氧化氮(NOx)浓度，并对SOD3基因Ala58Thr和eNOS基因Glu298Asp进行基因分型。进一步的关联实验在扩大的人群中重复进行，包括343名高血压患者和290名对照。结果表明，高血压病患者血浆总超氧化物歧化酶活性和NOx浓度与正常对照组比较差异无统计学意义。SOD3Ala/Ala纯合子携带者血浆超氧化物歧化酶活性(80.51±27.68U/ml)显著低于Thr等位基因携带者(92.18±16.37U/ml，P=0.031)。ENOS Glu/Glu纯合子携带者的血浆NOx浓度(129.66±59.15Mol/L)显著低于Asp等位基因携带者(169.84±55.18Mol/L；P=0.010)。值得注意的是，在80名参与者中，56.3%的人改变了SOD活性水平和NOx浓度。因此，高血压患者血清中SOD3Ala/Ala和/或eNOS Glu/Glu的基因分型与SOD活性和NOx浓度降低的一致性，可能有助于指导高血压患者的抗氧化治疗。
Oxidative stress, characterized by increased reactive oxygen species production and/or decreased antioxidant enzyme activity, plays an important role in the pathogenesis of hypertension. The identification of molecular markers corresponding to the oxidative stress status of hypertension may assist in the antioxidant therapy of hypertension. In the present study, superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS) were analyzed as markers of hypertension responding to oxidative stress. The plasma SOD activity and mononitrogen oxides (NOx) concentration were measured, and the SOD3 Ala58Thr and eNOS Glu298Asp polymorphisms were genotyped in hypertensive patients and normotensive controls. Further association experiments were replicated in an extended population, including 343 hypertensive patients and 290 controls. The results demonstrated that no statistically significant differences in the total SOD activity and NOx concentration were identified between the hypertensive patients and controls. However, the plasma SOD activity levels in the SOD3 Ala/Ala homozygote carriers (80.51±27.68 U/ml) were significantly lower compared with the Thr allele carriers (92.18±16.37 U/ml; P=0.031). In addition, the plasma NOx concentration in the eNOS Glu/Glu homozygote carriers (129.66±59.15 μmol/l) was significantly lower compared with the Asp allele carriers (169.84± 55.18 μmol/l; P=0.010). Notably, the altered SOD activity levels and NOx concentration were in concordance in 56.3% of the 80 participants. Therefore, the concordance of decreased SOD activity and NOx concentration, combined with genotypes of SOD3 Ala/Ala and/or eNOS Glu/Glu in hypertensive patients, may be useful in directing the antioxidant therapy of hypertension.
登录
查看更多内容
影响因子：
3.6
作者：
AUTOR, AP;FRANK, L;ROBERTS, RJ
通讯作者：
ROBERTS, RJ
影响因子：
4.8
作者：
Kuzkaya, N;Weissmann, N;Dikalov, S
通讯作者：
Dikalov, S
DOI：
10.1152/ajprenal.00060.2004
发表时间：
2004-11-01
影响因子：
4.2
作者：
Adler, S;Huang, H
通讯作者：
Huang, H
影响因子：
3.2
作者：
Benjafield, AV;Morris, BJ
通讯作者：
Morris, BJ
影响因子：
5.4
作者：
Naganuma, Takahiro;Nakayama, Tomohiro;Usami, Ron
通讯作者：
Usami, Ron