The pH dependence of motility and the acrosome reaction of guinea pig spermatozoa
The pH dependence of motility and the acrosome reaction of guinea pig spermatozoa
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豚鼠精子活力的pH依赖性和顶体反应
DOI：
10.1002/mrd.1120100102
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Yanagimachi
中科院分区：
文献类型：
--
作者：
S. J. Murphy;R. Yanagimachi
通过将完全获能的精子从无钙培养基转移到不同pH值（8.2、7.5、7.0、6.7、6.4和6.1）的含钙培养基中，研究了pH对豚鼠精子活力和顶体反应的影响。当转移到pH 7.5或8.2的培养基中时，精子在15-20分钟内进行顶体反应达到最大水平。顶体反应的精子是活跃的。精子活力和顶体反应的发生率随pH值的降低而降低。在pH 6.1时，所有精子的活动力迅速变得非常弱。没有顶体反应发生在这个低pH值。在pH值为6.1的运动性和顶体反应的抑制被逆转转移的精子回到碱性介质中，除非他们被暴露在这个低pH值超过3小时。顶体反应的抑制在低pH值（比6.1）在一定程度上被抵消增加浓度的Ca 2+（4-10 mM）。低pH可逆地抑制精子活力和顶体反应的可能机制进行了讨论。
The effect of pH on both motility and the acrosome reaction of guinea pig spermatozoa was examined by transferring fully capacitated spermatozoa from Ca2+ -free medium to Ca2+ -containing media with various pH values (8.2, 7.5, 7.0, 6.7, 6.4, and 6.1). When transferred to media of pH 7.5 or 8.2, the spermatozoa underwent their acrosome reactions to the maximum level within 15–20 min. Acrosome-reacted spermatozoa were vigorously motile. The intensity of sperm motility and the incidence of the acrosome reaction declined with decreasing pH values. At pH 6.1, the motility of all the spermatozoa quickly became very weak. No acrosome reactions occurred at this low pH. The inhibition of motility and the acrosome reaction at pH 6.1 was reversed by transferring the spermatozoa back to the alkaline medium unless they were exposed to this low pH for longer than 3 hr. The inhibition of the acrosome reaction at low pH values (other than 6.1) was counteracted to some extent by increased concentrations of Ca2+ (4–10 mM). Possible mechanisms by which low pH reversibly inhibits sperm motility and the acrosome reaction are discussed.
豚鼠精子在pH 7.8以下缓冲介质中获能和顶体反应所需的血清因子。
影响因子：
3.6
作者：
Hyne,RV;Garbers,DL
通讯作者：
Garbers,DL