Identification of Viral MicroRNAs Expressed in Human Sacral Ganglia Latently Infected with Herpes Simplex Virus 2
Identification of Viral MicroRNAs Expressed in Human Sacral Ganglia Latently Infected with Herpes Simplex Virus 2
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DOI：
10.1128/jvi.01712-09
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发表时间：
2010-01-15
影响因子：
5.4
通讯作者：
Cullen, Bryan R.
中科院分区：
文献类型：
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作者：
Umbach, Jennifer L.;Wang, Kening;Cullen, Bryan R.
对从单纯疱疹病毒2型（HSV-2）潜伏感染的人骶神经节中分离的小RNA进行深度测序，以鉴定潜伏感染期间表达的HSV-2 microRNA（miRNA）。这一努力导致了五种不同的HSV-2 miRNA种类的鉴定，其中两种，miR-H3/miR-I和miR-H4/miR-II，先前已报道。还鉴定了三种新的HSV-2 miRNA，其中两种miR-H7和miR-H9来源于潜伏相关转录物（LAT），并且位于编码反式激活因子ICP 0的病毒转录物的反义。第三种新的HSV-2 miRNA，miR-H10，在基因组的独特长（U-L）区域内编码，位于U（L）15开放阅读框的3'端，并且推测是从不同于LAT的新的潜伏HSV-2转录物中切除的。
Deep sequencing of small RNAs isolated from human sacral ganglia latently infected with herpes simplex virus 2 (HSV-2) was used to identify HSV-2 microRNAs (miRNAs) expressed during latent infection. This effort resulted in the identification of five distinct HSV-2 miRNA species, two of which, miR-H3/miR-I and miR-H4/miR-II, have been previously reported. Three novel HSV-2 miRNAs were also identified, and two of these, miR-H7 and miR-H9, are derived from the latency-associated transcript (LAT) and are located antisense to the viral transcript encoding transactivator ICP0. A third novel HSV-2 miRNA, miR-H10, is encoded within the unique long (U-L) region of the genome, 3' to the U(L)15 open reading frame, and is presumably excised from a novel, latent HSV-2 transcript distinct from LAT.