Genetic and molecular characterization of the maize rp3 rust resistance locus.
Genetic and molecular characterization of the maize rp3 rust resistance locus.
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发表时间：
2002-09
期刊：
影响因子：
3.3
通讯作者：
C. Webb;T. Richter;N. Collins;Marie Nicolas;H. Trick;T. Pryor;S. Hulbert
中科院分区：
文献类型：
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作者：
C. Webb;T. Richter;N. Collins;Marie Nicolas;H. Trick;T. Pryor;S. Hulbert
在玉米中，Rp 3基因赋予对由高粱柄锈菌引起的普通锈病的抗性。对感锈病的rp 3变异体的侧翼标记分析表明，它们中的大多数是通过不等交换产生的，表明rp 3是一个复杂的位点，如rp 1。PIC 13探针鉴定了映射到复合物的核苷酸结合位点-富含亮氨酸重复序列（NBS-LRR）基因家族。Rp 3变异体在用PIC 13探测时显示PIC 13家族成员相对于抗性亲本的损失，表明Rp 3基因是该家族的成员。凝胶印迹和序列分析表明，至少有9个家庭成员在大多数Rp 3携带线的基因座，其中至少有5个转录的Rp 3-A单倍型。14个家族成员的编码区，从三个不同的Rp 3携带单倍型分离，有93%至99%的DNA序列的同一性。对跨越玉米自交系B73中rp 3基因座的BAC重叠群的克隆进行部分测序，在约140 kb的区域中鉴定出5种不同的PIC 13旁系同源物。
In maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of approximately 140 kb.