Photoreceptor cell development requires prostaglandin signaling in the zebrafish retina
Photoreceptor cell development requires prostaglandin signaling in the zebrafish retina
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感光细胞发育需要斑马鱼视网膜中的前列腺素信号传导
DOI：
10.1016/j.bbrc.2019.01.073
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发表时间：
2019
影响因子：
3.1
通讯作者：
Zhong Tao P.
中科院分区：
文献类型：
--
作者：
Li Wenyan;Jin Daqing;Zhong Tao P.
光感受器细胞是高度特化的感觉神经元，能够进行视觉光传导。视网膜光感受器中的连接纤毛连接内、外视节，并介导视蛋白在视觉中的运输。虽然我们之前的研究表明前列腺素E2 （PGE2）信号是斑马鱼纤毛发生所必需的，但其在视网膜纤毛发生和光感受器细胞发育中的作用尚不清楚。在此，我们研究了前列腺素信号在视网膜和光感受器细胞发育中的作用。我们在斑马鱼中产生了前列腺素内过氧化物合成酶1 （PTGS1）和前列腺素内过氧化物合成酶2 （PTGS2）的突变，这两种限速酶负责前列腺素的产生。我们发现ptgs2block敲低ptgs1-/-ptgs2a-/-double突变体显著减少了连接纤毛的长度，导致光感受器细胞分化的严重缺陷。此外，pge2转运体漏尾(Lkt)/ atp结合盒转运体4 （ABCC4）突变导致光感受器细胞中连接纤毛和外段的缺失，导致视蛋白在光感受器细胞体中的错误定位。总之，我们的研究结果表明，pge2的产生和运输对于连接纤毛形成和光感受器细胞发育至关重要。
Photoreceptor cells are highly specialized sensory neurons capable of visual phototransduction. The connecting cilia in the retinal photoreceptors link the inner segment to the outer segment and mediate the transport of opsins in vision. Although our previous study demonstrates that Prostaglandin E2 (PGE2) signaling is required for ciliogenesis in zebrafish, its roles in retinal ciliogenesis and photoreceptor cell development remain unknown. Here, we investigated the function of prostaglandin signaling in retina and photoreceptor cell development. We generated zebrafish mutations in prostaglandin endoperoxide synthase 1 (PTGS1) and prostaglandin endoperoxide synthase 2 (PTGS2), two rate-limiting enzymes responsible for prostaglandin production. We found thatptgs2bknockdown inptgs1-/-ptgs2a-/-double mutants significantly reduced the length of connecting cilia and resulted in severe defects in photoreceptor cell differentiation. Furthermore, mutation in PGE2transporter Leakytail (Lkt)/ATP-binding cassette transporter 4 (ABCC4) exhibited loss of connecting cilia and outer segment in photoreceptor cells, leading to mislocalization of opsins in the cell bodies of photoreceptors. Together, our findings suggest that PGE2production and transport are crucial for connecting cilia formation and photoreceptor cell development.