Behaviors of Glioblastoma Cells in in Vitro Microenvironments
Behaviors of Glioblastoma Cells in in Vitro Microenvironments
复制标题
胶质母细胞瘤细胞在体外微环境中的行为
DOI：
10.1038/s41598-018-36347-7
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发表时间：
2019-01-14
影响因子：
4.6
通讯作者：
Ou-Yang, Zhong-Can
中科院分区：
文献类型：
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作者：
Diao, Wenwen;Tong, Xuezhi;Ou-Yang, Zhong-Can
胶质母细胞瘤（GBM）是最恶性和高度侵袭性的脑肿瘤。在这项研究中，四种典型的 GBM 细胞系（LN229、SNB19、U87、U251）在微型 3D 模型中培养，以研究它们的体外行为。 3-D 体外模型提供包含天然胶原界面的中空微室阵列，从而允许 GBM 细胞在 3-D 室中生长。该模型中的GBM细胞在细胞形态、增殖、迁移和侵袭方面表现出特定的特性，其中一些特性是以前很少观察到的。此外，详细观察了细胞如何侵入周围的ECM以及相应的特定侵入模式，这意味着这四种细胞在癌症发展过程中具有不同的特征。这种复杂的体外模型如果应用于患者来源的细胞，就有可能成为临床相关的预测模型。
Glioblastoma (GBM) is the most malignant and highly aggressive brain tumor. In this study, four types of typical GBM cell lines (LN229, SNB19, U87, U251) were cultured in a microfabricated 3-D model to study their in vitro behaviors. The 3-D in vitro model provides hollow micro-chamber arrays containing a natural collagen interface and thus allows the GBM cells to grow in the 3-D chambers. The GBM cells in this model showed specific properties on the aspects of cell morphology, proliferation, migration, and invasion, some of which were rarely observed before. Furthermore, how the cells invaded into the surrounding ECM and the corresponding specific invasion patterns were observed in details, implying that the four types of cells have different features during their development in cancer. This complex in vitro model, if applied to patient derived cells, possesses the potential of becoming a clinically relevant predictive model.