A Streptococcus suis strain Δcps/ssna-mSLY (P353L)-SC19 provides cross-protection against serotype 2 and 9 strain challenges in mice model
A Streptococcus suis strain Δcps/ssna-mSLY (P353L)-SC19 provides cross-protection against serotype 2 and 9 strain challenges in mice model
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作者：
Lu Xi;Xu Lei;Lin Lan;Zhou Liting;Dai Bingqian;Cui Shuyue;Zhang Anding
Streptococcus suis is an important zoonotic pathogen that mainly causes meningitis, septicemia, and arthritis. Due to the limited cross-protection between numerous serotypes, the existing inactive vaccines in clinical use fail to offer sufficient protection. In this study, a gene deletion-attenuated strain Δcps/ssna-msly (P353L)-SC-19 was constructed by deleting cps and ssna genes from the epidemic strain SC-19 with a mutation of SLY (P353L). The safety of Δcps/ssna-msly (P353L)-SC-19 was confirmed in both in vitro and in vivo experiments. We further demonstrated that immunization with Δcps/ssna-msly (P353L)-SC-19 induced significant cellular immunity and humoral immunity in mice and protected against infections caused by type 2 strain SC-19 (100% protection) and type 9 strain S29 (50% protection), while also preventing meningitis induced by S29. This study highlights the potential of using CPS-deficient strains to achieve cross-protection against different Streptococcus suis serotypes and develop a promising universal live vaccine.
