Amelioration of Benzo[a]pyrene-induced oxidative stress and pulmonary toxicity by Naringenin in Wistar rats: A plausible role of COX-2 and NF-κB
Amelioration of Benzo[a]pyrene-induced oxidative stress and pulmonary toxicity by Naringenin in Wistar rats: A plausible role of COX-2 and NF-κB
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DOI：
10.1177/0960327116650009
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发表时间：
2017-04-01
影响因子：
2.8
通讯作者：
Sultana, S.
中科院分区：
文献类型：
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作者：
Ali, R.;Shahid, A.;Sultana, S.
柚皮素是一种天然存在的黄烷酮类化合物，具有清除自由基、抑制酶、抗氧化、抗炎和抗癌等作用。本研究旨在评价柚皮素对苯并[a]芘（B[a]P）诱导的氧化应激和肺毒性的保护作用。用剂量为100 mg/kg体重的柚皮素处理大鼠（B. wt.），口服强饲法B[a]P 50 mg/kg单次给药B。重量是腹腔注射的通过检测总蛋白、总细胞计数、乳酸脱氢酶、脂质过氧化、还原型谷胱甘肽、抗氧化酶活性、肺组织学以及核因子κ B（NF-kB B）和环氧合酶-2（考克斯-2）的表达来评价柚皮素的保护作用。组织病理学和免疫组化观察肺毒性和炎症反应。B[a]P给药提高了肺损伤标志物的水平，降低了抗氧化酶的活性。柚皮素治疗通过恢复抗氧化酶来减弱氧化应激水平，进一步改善肺组织学损伤并显著降低炎症反应。柚皮素还有效地降低了由B[a] P诱导的NF-κ B和考克斯-2的表达。这些发现表明，柚皮素补充有利于维持肺泡和上皮的完整性，其可用作B[a] P诱导的氧化应激和肺损伤的保护剂。然而，需要进一步的研究来阐明柚皮素的潜在作用机制。
Naringenin is a naturally occurring flavanones and has been found to exhibit free radical scavenging, enzyme inhibition, antioxidants, anti-inflammatory, and anticancer activities. Present study was designed to evaluate the protective role of naringenin against benzo[a]pyrene (B[a]P)-induced oxidative stress and pulmonary toxicity. Rats were treated with naringenin at a dose of 100 mg/kg body weight (b. wt.), by oral gavage. B[a]P in a single dose of 50 mg/kg b. wt. was given intraperitoneally. Total protein, total cell counts, lactate dehydrogenase, lipid peroxidation, reduced glutathione, antioxidant enzymes activities, lung histology and expression of nuclear factor kappa B (NF-kB), and cyclo-oxygenase-2 (COX-2) was assessed to evaluate protective effects of naringenin. Histopathological and immunohistochemical studies were also carried out to observe lung toxicity and inflammation. B[a]P administration enhanced the levels of lung injury markers and reduced antioxidant enzymes activities. Naringenin treatment attenuated the levels of oxidative stress by restoring antioxidant enzymes, further improved lung histological damage and significant decrease in inflammatory responses. Naringenin also effectively decreased the expression of NF-kB, and COX-2 induced by B[a]P. These findings suggest that naringenin supplementation is beneficial in maintaining the integrity of alveoli and the epithelium that may be used as a protective agent in B[a]P-induced oxidative stress and lung damage. However, further studies are warranted to elucidate the potential mechanism of action of naringenin.