Spatial organization of silybin biosynthesis in milk thistle [Silybum marianum (L.) Gaertn]
Spatial organization of silybin biosynthesis in milk thistle [Silybum marianum (L.) Gaertn]
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水飞蓟素生物合成的空间组织 [Silybum marianum (L.) Gaertn]
DOI：
10.1111/tpj.13736
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发表时间：
2017-12-01
期刊：
影响因子：
7.2
通讯作者：
Chen, Jian
中科院分区：
文献类型：
--
作者：
Lv, Yongkun;Gao, Song;Chen, Jian
水莲蛋白是从药用牛奶蓟中提取的化合物的集合，其中甲硅烷基是主要的黄酮质。然而，尚不清楚的是，硅鸟的生物合成途径尚不清楚。在这项研究中，仿生反应表明，通过过氧化物酶的作用，可以从针叶醇和出租车蛋白合成甲硅豆蛋白。硅鸟蛋白及其前体的浓度谱以及基因表达的RNA-seq分析表明，出租车蛋白的量和过氧化物酶的活性是甲硅烷基素生物合成的限制因子。类黄酮生物合成途径的基因表达谱的分层聚类将花与其他器官区分开。 RNA-seq揭示了参与甲硅烷基蛋白产生的过氧化物酶的五个候选者，其中APX1（抗坏血酸酯过氧化物酶1）显示出明显的过氧化物酶活性和合成甲硅豆蛋白的能力。阐明了牛奶蓟中硅鸟生物合成的空间组织，这可以帮助我们理解甲硅烷基宾和其他黄酮基因的生物合成。
Silymarin is a collection of compounds extracted from the medicinal herb milk thistle, among which silybin is the major flavonolignan. However, the biosynthesis pathway of silybin remains unclear. In this study, biomimetic reactions demonstrated that silybin can be synthesized from coniferyl alcohol and taxifolin by the action of peroxidase. The concentration profiles of silybin and its precursors and RNA-Seq analysis of gene expression revealed that the amount of taxifolin and the activity of peroxidase serve as the limiting factors in silybin biosynthesis. Hierarchical clustering of the expression profile of genes of the flavonoid biosynthesis pathway distinguished flowers from other organs. RNA-Seq revealed five candidates for the peroxidase involved in silybin production, among which APX1 (ascorbate peroxidase 1) showed a distinct peroxidase activity and the capacity to synthesize silybin. The spatial organization of silybin biosynthesis in milk thistle was elucidated, which could help our understanding of the biosynthesis of silybin and other flavonolignans.