Identification of phyllostine as an intermediate of the patulin pathway in Penicillium urticae.
Identification of phyllostine as an intermediate of the patulin pathway in Penicillium urticae.
复制标题
鉴定叶绿素是二斑青霉中棒曲霉素途径的中间体。
作者：
J. Sekiguchi;G. M. Gaucher
发现二斑青霉 (NRRL 2159A) 的棒曲霉素阴性突变体 (J1) 积累了大量（大于 128 mg/L 培养物）的反应性光敏化合物，该化合物被分离并鉴定为 (-)-叶绿素（5,6-环氧龙胆基醌）。这种环氧醌对枯草芽孢杆菌具有抗菌活性，约为棒曲霉素的 80%。在单独的体内喂养实验中，[2-14C]乙酸和[G-3H]龙胆醛很容易被突变体J1掺入叶绿素中，并且[14C]叶绿素被亲本菌株（NRRL 2159A）掺入展曲霉素中。当将产生龙胆醛但不产生叶绿素的第二展青霉素阴性突变体 (J2) 的洗涤细胞悬浮液喂入时，未标记的叶绿素在 30 分钟、1 和 5 小时后有效转化为展青霉素，产率分别为 33%、56% 和 92%。讨论了叶绿素作为展青霉素生物合成途径新的后龙胆醛部分的中间体的作用。
A patulin negative mutant (J1) of Penicillium urticae (NRRL 2159A) was found to accumulate large quantities (greater than 128 mg/L culture) of a reactive, photosensitive compound, which was isolated and identified as (-)-phyllostine (5,6-epoxygentisylquinone). This epoxyquinone possessed an antibiotic activity against Bacillus subtilis which was approximately 80% of that exhibited by patulin. In separate in vivo feeding experiments, [2-14C]acetate and [G-3H]gentisaldehyde were readily incorporated into phyllostine by mutant J1 and [14C]phyllostine was incorporated into patulin by the parent strain (NRRL 2159A). When fed to a washed-cell suspension of a second patulin negative mutant (J2) which produced gentisaldehyde but not phyllostine, unlabeled phyllostine was efficiently converted to patulin in yields of 33, 56, and 92% after 30 min, 1 and 5 h, respectively. The role of phyllostine as an intermediate of a new post-gentisaldehyde portion of the patulin biosynthetic pathway is discussed.