A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum
A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum
复制标题
DOI：
10.1016/j.molbiopara.2008.10.004
复制
发表时间：
2009-02-01
影响因子：
1.5
通讯作者：
Trenholme, Katharine R.
中科院分区：
文献类型：
--
作者：
Dixon, Matthew W. A.;Peatey, Christopher L.;Trenholme, Katharine R.
疟疾寄生虫性血阶段形成的研究由于缺乏一种可靠的、可重复的、没有操作者偏倚和误差的测定方法而受到严重阻碍。在这里，我们报告的发展，利用绿色荧光蛋白嵌合体的早期性血液阶段蛋白Pfs16作为一个标记的承诺配子体发生的测定。通过荧光激活细胞分选的寄生虫分析允许在形态学变化明显之前准确评估配子体产生。(C)2008 Elsevier B.V.保留所有权利。
Study of the formation of the sexual blood stages of the malaria parasite has been significantly hampered by the absence of a reliable, reproducible assay devoid of operator bias and error. Here we report on the development of an assay utilizing a green fluorescent protein chimera of the early sexual blood stage protein Pfs16 as a marker for commitment to gametocytogenesis. Analysis of parasites via fluorescence activated cell sorting allows for the accurate assessment of gametocyte production well before morphological changes are apparent. (C) 2008 Elsevier B.V. All rights reserved.