Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo
Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo
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DOI：
10.1038/sj.gt.3301173
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发表时间：
2000-05-01
期刊：
影响因子：
5.1
通讯作者：
Heller, R
中科院分区：
文献类型：
--
作者：
Heller, L;Jaroszeski, MJ;Heller, R
通过直接递送质粒DNA的基因治疗具有优于病毒基因转移的几个优点，但质粒递送的效率较低。在动物和人的研究中，体内电穿孔已被用于增强化疗剂向肿瘤的递送。最近，这种递送技术已扩展到大分子，如质粒DNA。在这里，成功交付的质粒编码报告基因的大鼠肝细胞癌的体内电穿孔证明。
Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. in vivo electroporation has been used to enhance delivery of chemotherapeutic agents to tumors in both animal and human studies. Recently, this delivery technique has been extended to large molecules such as plasmid DNA. Here, the successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated.