Conserved architecture of the core RNA polymerase II initiation complex
Conserved architecture of the core RNA polymerase II initiation complex
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DOI：
10.1038/ncomms5310
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发表时间：
2014-07-01
影响因子：
16.6
通讯作者：
Cramer, Patrick
中科院分区：
文献类型：
--
作者：
Muehlbacher, Wolfgang;Sainsbury, Sarah;Cramer, Patrick
在蛋白质编码基因启动子的转录起始过程中，RNA聚合酶（Pol）II与TBP、TFIIB和TFIIF组装成保守的核心起始复合物，该复合物募集额外的因子。核心复合物稳定开放DNA并启动RNA合成，并且它在Pol I和Pol III转录系统中是保守的。在这里，我们得到的结构域架构的酵母核心聚合酶II起始复合物在转录起始。酵母复合物类似于人类起始复合物，并揭示了TFIIF Tfg 2翼螺旋结构域在启动子DNA上摆动。TFIIF中的“臂”和“带电螺旋”分别在转录起始位点选择和初始RNA合成中起作用，并且显然延伸到活性中心裂缝中。我们的模型提供了进一步的结构-功能分析的基础上，整个转录起始复合物。
During transcription initiation at promoters of protein-coding genes, RNA polymerase (Pol) II assembles with TBP, TFIIB and TFIIF into a conserved core initiation complex that recruits additional factors. The core complex stabilizes open DNA and initiates RNA synthesis, and it is conserved in the Pol I and Pol III transcription systems. Here, we derive the domain architecture of the yeast core pol II initiation complex during transcription initiation. The yeast complex resembles the human initiation complex and reveals that the TFIIF Tfg2 winged helix domain swings over promoter DNA. An 'arm' and a 'charged helix' in TFIIF function in transcription start site selection and initial RNA synthesis, respectively, and apparently extend into the active centre cleft. Our model provides the basis for further structure-function analysis of the entire transcription initiation complex.