Structure-activity relationships of the ATP cofactor in ligase-catalysed oligonucleotide polymerisations.
Structure-activity relationships of the ATP cofactor in ligase-catalysed oligonucleotide polymerisations.
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连接酶催化寡核苷酸聚合中 ATP 辅因子的构效关系。
DOI：
10.1039/c6ob02792j
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发表时间：
2017
影响因子：
3.2
通讯作者：
Hili,Ryan
中科院分区：
文献类型：
--
作者：
Lei,Yi;Hili,Ryan
最近开发了一种 T4 DNA 连接酶催化的寡核苷酸聚合工艺，能够在整个核酸聚合物中掺入多个官能团。 T4 DNA 连接酶在聚合过程中需要 ATP 作为辅助因子来催化磷酸二酯键的形成。在此，我们描述了 T4 DNA 连接酶催化寡核苷酸聚合背景下 ATP 的构效关系。使用高通量测序，我们不仅研究了 ATP 修饰对聚合效率的影响，而且还研究了聚合过程的保真度和序列偏差。
A T4 DNA ligase-catalysed oligonucleotide polymerisation process has been recently developed to enable the incorporation of multiple functional groups throughout a nucleic acid polymer. T4 DNA ligase requires ATP as a cofactor to catalyse phosphodiester bond formation during the polymerisation process. Herein, we describe the structure–activity relationship of ATP within the context of T4 DNA ligase-catalyzed oligonucleotide polymerisation. Using high-throughput sequencing, we study not only the influence of ATP modification on polymerisation efficiency, but also on the fidelity and sequence bias of the polymerisation process.