Changes in the expression and localization of cohesin subunits during meiosis in a non-mammalian vertebrate, the medaka fish
Changes in the expression and localization of cohesin subunits during meiosis in a non-mammalian vertebrate, the medaka fish
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DOI：
10.1016/j.modgep.2004.03.004
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发表时间：
2004-09-01
影响因子：
1.2
通讯作者：
Yamashita, M
中科院分区：
文献类型：
--
作者：
Iwai, T;Lee, J;Yamashita, M
直到后期，姐妹染色单体通过一种称为粘附素的多亚基蛋白质复合体相互结合。由于减数分裂中染色体的行为与有丝分裂中的不同，减数分裂中同源染色体和姐妹染色单体的结合和分离被认为是由减数分裂特有的粘附素亚基调节的。事实上，已知在哺乳动物中存在几个减数分裂特异的粘附素亚基，包括Rec8、STAG3和SMC1beta；然而，在其他脊椎动物中还没有关于减数分裂特异的粘附素亚基的报道。为了研究非哺乳动物物种减数分裂过程中粘附素亚基的蛋白表达和定位，我们从青竹中分离了编码SMC1a、SMC1beta、SMC3和Rad21的基因克隆，并制备了抗重组蛋白的抗体。青竹SMC1beta仅在性腺中表达，SMC1pha、SMC3和Rad21也在其他器官和培养细胞中表达。SMC1beta与SMC3形成络合物，但不与Rad21形成络合物，而SMC1pha与SMC3和Rad21形成络合物。SMC1pha和Rad21主要表达于睾丸中有丝分裂的细胞(体细胞和精原细胞)，但在细线期前期精母细胞中表达较弱。SMC1beta在精原细胞和精母细胞中有表达。SMC1beta定位于减数分裂前期1的染色体臂和着丝粒上，并持续到中期H，这与在小鼠中报道的情况不同，在粗线期晚期，SMC1beta从染色体臂上消失，尽管它在脊椎动物中普遍存在。(C)2004爱思唯尔B.V.保留所有权利。
Until the onset of anaphase, sister chromatids are bound to each other by a multi-subunit protein complex called cohesin. Since chromosomes in meiosis behave differently from those in mitosis, the cohesion and separation of homologous chromosomes and sister chromatids in meiosis are thought to be regulated by meiosis-specific cohesin subunits. Actually, several meiosis-specific cohesin subunits, including Rec8, STAG3 and SMC1beta, are known to exist in mammals; however, there are no reports of meiosis-specific cohesin subunits in other vertebrates. To investigate the protein expression and localization of cohesin subunits during meiosis in non-mammalian species, we isolated cDNA clones encoding SMC1alpha, SMC1beta, SMC3 and Rad21 in the medaka and produced antibodies against recombinant proteins. Medaka SMC1beta was expressed solely in gonads, while SMC1alpha, SMC3 and Rad21 were also expressed in other organs and in cultured cells. SMC1beta forms a complex with SMC3 but not with Rad21, in contrast to SMC1alpha, which forms complexes with both SMC3 and Rad21. SMC1alpha and Rad21 were mainly expressed in mitotically dividing cells in the testis (somatic cells and spermatogonia), although their weak expression was detected in pre-leptotene spermatocytes. SMC1beta was expressed in spermatogonia and spermatocytes. SMC1beta was localized along the chromosomal arms as well as on the centromeres in meiotic prophase 1, and its existence on the chromosomes persisted up to metaphase H, a situation different from that reported in the mouse, in which SMC1beta is lost from the chromosome arms in late pachytene despite its universal presence in vertebrates. (C) 2004 Elsevier B.V. All rights reserved.