A DEFECTIVE HSV-1 VECTOR EXPRESSES ESCHERICHIA-COLI BETA-GALACTOSIDASE IN CULTURED PERIPHERAL NEURONS
A DEFECTIVE HSV-1 VECTOR EXPRESSES ESCHERICHIA-COLI BETA-GALACTOSIDASE IN CULTURED PERIPHERAL NEURONS
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DOI：
10.1126/science.2843986
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发表时间：
1988-09-23
期刊：
影响因子：
56.9
通讯作者：
BREAKEFIELD, XO
中科院分区：
文献类型：
--
作者：
GELLER, AI;BREAKEFIELD, XO
已经开发了缺陷型单纯疱疹病毒1（HSV-1）载体pHSVlac，其含有将大肠杆菌lacZ基因置于HSV-1立即早期4/5启动子控制下的转录单位。载体pHSVlac用HSV-1温度敏感突变体ts K作为辅助病毒繁殖。在原代培养物中感染来自大鼠上级颈神经节和背根神经节的神经元导致高水平的β-半乳糖苷酶而无细胞死亡。这些HSV-1载体应该是有用的有丝分裂后的细胞，如神经元，在体外和体内引入基因。
A defective herpes simplex virus 1 (HSV-1) vector, pHSVlac, has been developed that contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. The vector pHSVlac was propagated with the HSV-1 temperature-sensitive mutant ts K as helper virus. Infection of neurons from rat superior cervical ganglia and dorsal root ganglia in primary culture resulted in stable expression of high levels of .beta.-galactosidase without cell death. These HSV-1 vectors should be useful for introducing genes into postmitotic cells, such as neurons, in vitro and in vivo.