MoS2 nanosheet-based fluorescent biosensor for protein detection via terminal protection of small-molecule-linked DNA and exonuclease III-aided DNA recycling amplification
MoS2 nanosheet-based fluorescent biosensor for protein detection via terminal protection of small-molecule-linked DNA and exonuclease III-aided DNA recycling amplification
复制标题
基于 MoS2 纳米片的荧光生物传感器，通过小分子连接 DNA 的末端保护和核酸外切酶 III 辅助的 DNA 回收扩增来检测蛋白质
DOI：
10.1016/j.bios.2015.06.045
复制
发表时间：
2015-12-15
影响因子：
12.6
通讯作者：
Xu, Jiqu
中科院分区：
文献类型：
--
作者：
Xiang, Xia;Shi, Jianbin;Xu, Jiqu
研制了一种新型的基于MoS2纳米片的蛋白质荧光生物传感器。该方法结合了小分子连锁DNA的末端保护(TPSMLD)和外切酶III(Exo III)辅助的DNA循环扩增，将蛋白质分析转化为对DNA的高灵敏度检测。以链霉亲和素(SA)-生物素体系为模型，得到SA的检出限为0.67 ng mL(-1)，且具有良好的选择性。本研究不仅为蛋白质检测提供了简单、灵敏、选择性强的检测方法，而且将拓展新兴的二维纳米材料在生物检测中的应用。(C)2015爱思唯尔B.V.保留所有权利。
A new MOS2 nanosheet-based fluorescent biosensor for protein detection is developed. This method combines the terminal protection of small-molecule-linked DNA (TPSMLD) and exonuclease III (Exo III)aided DNA recycling amplification to convert protein assay into the highly sensitive detection of DNA. Taking the streptavidin (SA)-biotin system as a model, a detection limit of 0.67 ng mL(-1) SA is obtained with a good selectivity. The study demonstrated here not only offers simple, sensitive and selective detection method for protein assay, but also will expand the application of the emerging 2D nanomaterials into biological assay. (C) 2015 Elsevier B.V. All rights reserved.