On Plasma Fractionation Treatment and Its Implications in Cells
On Plasma Fractionation Treatment and Its Implications in Cells
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DOI：
10.1109/trpms.2022.3178286
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发表时间：
2023-01
影响因子：
4.4
通讯作者：
Nishtha Gaur;R. Short;S. Allinson
中科院分区：
文献类型：
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作者：
Nishtha Gaur;R. Short;S. Allinson
在此，我们提出了一种新的等离子体治疗方案-等离子体分割，类似于放射治疗中的剂量分割概念，可以看到在基于等离子体的癌症治疗中的应用。在血浆分级中，将单次急性剂量的血浆分成多个小剂量（分级剂量），并以24小时的间隔在体外给予细胞。我们利用氦等离子体射流，并研究了永生化角质形成细胞系（HaCaT）和鳞状细胞癌细胞系（A431）中的血浆分馏的影响。在三种细胞接种密度-8000、3500和1000个细胞/孔下评估效果。我们的结果表明，在所有接种密度下，与作为单一血浆处理施用的相同剂量相比，血浆分级分离在两种细胞类型中产生较低水平的细胞死亡。这突出了血浆分离作为未来进行血浆治疗的潜在更安全方法的潜力。我们还表明，A431细胞比HaCaT细胞对单一急性血浆处理更敏感，细胞密度为亚融合（1000个细胞/孔）。HaCaT细胞和A431细胞之间的敏感性类似的差异，没有观察到外源性处理过氧化氢，指出其他较短的血浆成分的重要性。
Here, we present a novel plasma treatment regime—plasma fractionation, analogous to the concept of dose fractionation in radiotherapy, which could see the application in plasma-based cancer treatment. In plasma fractionation, a single acute dose of plasma is divided into multiple small dosages (fractionated dosages) and administered to the cells in vitro at 24-h intervals. We utilized a helium plasma jet and studied the effects of plasma fractionation in an immortalized keratinocyte line (HaCaT) and a squamous cell carcinoma line (A431). The effects were assessed over three cell seeding densities—8000, 3500, and 1000 cells/well. Our results show that at all seeding densities, plasma fractionation produced lower levels of cell death in both cell types compared to the same dose administered as a single plasma treatment. This highlights the potential of plasma fractionation as a potentially safer method to conduct plasma treatments in the future. We also show that A431 cells were more sensitive to a single acute plasma treatment than HaCaT cells, at cell densities that are subconfluent (1000 cells/well). A similar difference in sensitivity between HaCaT cells and A431 cells was not observed on exogenous treatment with hydrogen peroxide, pointing to the importance of other shorter lived plasma components.