Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor
Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor
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DOI：
10.1016/j.biortech.2011.04.078
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发表时间：
2011-07-01
影响因子：
11.4
通讯作者：
Shen, Yin-Chu
中科院分区：
文献类型：
--
作者：
Hu, Zhong-Ce;Zheng, Yu-Guo;Shen, Yin-Chu
利用氧化葡糖酵母细胞的甘油脱氢酶转化甘油可生产1，3-二羟基丙酮。首先，通过培养基优化提高甘油脱氢酶的活性。细胞培养的最佳培养基由5.6 g/l酵母提取物、4.7 g/l甘油、42.1 g/l甘露醇、0.5 g/l K2 HPO 4、0.5 g/l KH 2 PO 4、0.1 g/l MgSO 4中心点7 H（2）O和2.0 g/l CaCO 3组成，初始pH为4.9。其次，利用内循环气升式生物反应器，利用G. oxydans ZJB 09113.此外，pH值，通气量和细胞含量的DHA生产和甘油补料策略的影响进行了研究。在30 ℃、pH 5.0和1.5 vvm通气速率下，使用10 g/l静息细胞进行生物转化72 h后，获得156.3 +/- 7.8 g/l的最大DNA浓度和89.8 +/- 2.4%的甘油至DHA的转化率。(C)2011爱思唯尔有限公司保留所有权利。
1,3-Dihydroxyacetone can be produced by biotransformation of glycerol with glycerol dehydrogenase from Cluconobacter oxydans cells. Firstly, improvement the activity of glycerol dehydrogenase was carried out by medium optimization. The optimal medium for cell cultivation was composed of 5.6 g/l yeast extract, 4.7 g/l glycerol, 42.1 g/l mannitol, 0.5 g/l K2HPO4, 0.5 g/l KH2PO4, 0.1 g/l MgSO4 center dot 7H(2)O, and 2.0 g/l CaCO3 with the initial pH of 4.9. Secondly, an internal loop airlift bioreactor was applied for DNA production from glycerol by resting cells of G. oxydans ZJB09113. Furthermore, the effects of pH, aeration rate and cell content on DHA production and glycerol feeding strategy were investigated. 156.3 +/- 7.8 g/l of maximal DNA concentration with 89.8 +/- 2.4% of conversion rate of glycerol to DHA was achieved after 72 h of biotransformation using 10 g/l resting cells at 30 degrees C, pH 5.0 and 1.5 vvm of aeration rate. (C) 2011 Elsevier Ltd. All rights reserved.