Optical imaging of transferrin targeted PEI/DNA complexes in living subjects
Optical imaging of transferrin targeted PEI/DNA complexes in living subjects
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DOI：
10.1038/sj.gt.3301939
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发表时间：
2003-05-01
期刊：
影响因子：
5.1
通讯作者：
Gambhir, SS
中科院分区：
文献类型：
--
作者：
Hildebrandt, IJ;Iyer, M;Gambhir, SS
非侵入性光学生物发光成像系统是评估活体基因表达的重要工具，用于研究活体动物个体和时间变化。在这份报告中，我们证明了转铁蛋白（Tf）靶向聚乙烯亚胺（PEI）复合物，或没有，聚（乙二醇）（PEG）的修改，可以在活的A/J小鼠携带N2 A肿瘤使用冷却电荷耦合器件（CCD）相机成像的萤火虫荧光素酶报告基因（FL）的表达。尾静脉注射Tf-PEI-PEG、Tf-PE 1和PEI（阳性对照）复合物，并在复合物注射后5、24、48和72 h对小鼠进行成像。成像后，离体分析器官的萤火虫荧光素酶蛋白（FL）活性。Tf和PEG修饰的制剂显示显著（P
Noninvasive optical bioluminescence imaging systems are important tools for evaluating gene expression in vivo for study of individual and temporal variation in a living animal. In this report, we demonstrate that expression of the firefly luciferase reporter gene (fl) delivered by transferrin (Tf) targeted polyethylenimine (PEI) complexes with, or without, poly(ethylene glycol) (PEG) modifications can be imaged in living A/J mice bearing N2A tumors using a cooled charged coupled device (CCD) camera. Tf-PEI-PEG, Tf-PE1, and PEI (positive control) complexes were tail-vein injected and mice were imaged at 5, 24, 48, and 72 h after complex injection. After imaging, the organs were analyzed ex vivo for firefly luciferase protein (FL) activity. The Tf and PEG modified formulations show significantly (P