Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli
Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli
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DOI：
10.1271/bbb.60024
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发表时间：
2006-07-01
影响因子：
1.6
通讯作者：
Ishihama, Akira
中科院分区：
文献类型：
--
作者：
Yamamoto, Kaneyoshi;Ishihama, Akira
大肠杆菌中的铜刺激子由四种调节子组成，CueR-、CusS/CusR-、CpxA/CpxR-和YedV/YedW调节子。E. cpxRA缺陷的大肠杆菌突变体对铜的敏感性高于野生型。在E.在CpxA/CpxR依赖的方式暴露于铜的大肠杆菌培养物。凝胶位移和DNA酶I足迹分析后，五核苷酸序列的保守串联重复序列GTAAA（N）（4-8）GTAAA，每个五聚体上游4-bp的保守A，被鉴定为CpxR结合位点。CpxR盒的方向和位置的差异进行了讨论相对于CpxR-调节子基因之间的调节机制。
The copper stimulon in Escherichia coli consists of four regulons, the CueR-, CusS/CusR-, CpxA/CpxR-, and YedV/YedW regulons. E. coli mutants defective in cpxRA showed higher sensitivity to copper than the wild type. A total of 15 promoters were found to be induced in E. coli culture upon exposure to copper in a CpxA/ CpxR-dependent manner. After gel-shift and DNase I foot-printing analyses, a conserved tandem repeat of pentanucleotide sequence, GTAAA(N)(4-8)GTAAA, with a conserved A of 4-bp upstream of each pentamer, was identified to be the CpxR-binding site. The difference in the orientation and location of the CpxR box is discussed with respect to the regulation mechanism among CpxR-regulon genes.