Evaluation of rhizosphere bacterial antagonists for their potential to bioprotect potato (Solanum tuberosum) against bacterial wilt (Ralstonia solanacearum)
Evaluation of rhizosphere bacterial antagonists for their potential to bioprotect potato (Solanum tuberosum) against bacterial wilt (Ralstonia solanacearum)
复制标题
DOI：
10.1016/j.biocontrol.2008.09.003
复制
发表时间：
2008-12-01
影响因子：
4.2
通讯作者：
Hiskias, Yaynu
中科院分区：
文献类型：
--
作者：
Aliye, Naser;Fininsa, Chemeda;Hiskias, Yaynu
青枯病（Ralstonia solanacearum）是马铃薯（Solanum tuberosum）生产的制约因素之一。该研究的目的是评估细菌拮抗剂抑制青枯病发展的潜力，并评估这些菌株作为马铃薯植物根际促进生长细菌（PGPR）的作用。针对青枯雷尔斯顿氏菌 PPRC-Rs 的强毒株筛选了一百二十个根际细菌分离株。经过体外筛选，筛选出抑制直径>11 mm的6个拮抗菌株（PFMRI、BS-DFS、PF9、PF20、BC和BS-wly），并在温室中进行体内进一步研究。在体内研究中，从疾病进展曲线下面积（AUDPC）和增加马铃薯生物量（株高和干重）方面评估了菌株抑制疾病发展的效果。因此，与对照相比，PFMRI、BS-DFS 和 PF9 使 AUDPC 显着降低 78.6%、66% 和 64.3%，枯萎率显着降低 82.7%、66.2% 和 65.7%。在单独施用期间，与未细菌对照相比，这些菌株显着（P < 0.0001）使株高分别增加了 35.6%、45.9% 和 45%，干物质分别增加了 111%、130.4% 和 129%。在存在病原体菌株 PFMRI、BS-DFS 和 PF9 的情况下，与病原体处理的对照相比，株高分别增加了 66%、50% 和 48.2%，干物质分别增加了 153.8%、96.8% 和 92.5%。因此，研究表明 PFMRI、BS-DFS 和 PF9 菌株在马铃薯生物保护中具有潜在用途，如 PGPR 或综合青枯病管理；应研究其在各种现场条件下的有效性。 (C) 2008 Elsevier Inc. 保留所有权利。
Bacterial wilt (Ralstonia solanacearum) is one of the production constraints of potato (Solanum tuberosum). The intent of the study was to evaluate potential of bacteria] antagonists to suppress bacterial wilt disease development and evaluate the role of the strains as plant growth-promoting rhizobacteria (PGPR) in potato. One hundred-twenty rhizosphere bacteria] isolates were screened against virulent strain of Ralstonia solanacearum PPRC-Rs. After in vitro screening, six antagonistic strains (PFMRI, BS-DFS, PF9, PF20, BC, and BS-wly) with inhibition diameter >11 mm were selected and studied further in the greenhouse, in vivo. During in vivo study, the strains were evaluated for their effect in suppressing disease development in terms of area under disease progress curve (AUDPC) and increasing biomass (plant height and dry weight) of potato. Accordingly, PFMRI, BS-DFS, and PF9, significantly reduced AUDPC by 78.6, 66, and 64.3%, and wilt incidence by 82.7, 66.2, and 65.7%, respectively, compared to the control. During the sole application, the strains significantly (P < 0.0001) increased plant height by 35.6, 45.9, and 45%, and dry matter by 111, 130.4, and 129%, respectively compared to non-bacterized control. In the presence of the pathogen strain PFMRI, BS-DFS, and PF9 increased plant height by 66, 50, and 48.2%, and dry matter by 153.8, 96.8, and 92.5%, respectively compared to the pathogen treated control. Hence, the study shows that PFMRI, BS-DFS, and PF9 strains have potential use in potato bioprotection, as PGPR or in an integrated bacterial wilt management; whose effectiveness under a variety of field conditions should be investigated. (C) 2008 Elsevier Inc. All rights reserved.