Probing mechanisms for bioaccumulation of perfluoroalkyl acids in carp (Cyprinus carpio): Impacts of protein binding affinities and elimination pathways
Probing mechanisms for bioaccumulation of perfluoroalkyl acids in carp (Cyprinus carpio): Impacts of protein binding affinities and elimination pathways
复制标题
探究鲤鱼（Cyprinus carpio）中全氟烷基酸生物累积的机制：蛋白质结合亲和力和消除途径的影响
DOI：
10.1016/j.scitotenv.2018.08.099
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发表时间：
2019
影响因子：
9.8
通讯作者：
Zhu Lingyan
中科院分区：
文献类型：
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作者：
Zhong Wenjue;Zhang Liyuan;Cui Yannan;Chen Meng;Zhu Lingyan
随着对全氟辛酸盐（PFOA）、全氟辛烷磺酸盐（PFOS）和相关化合物的生产和使用的监管，短链全氟烷基酸（PFAAs）越来越多地被用作替代品。然而，关于其生物累积机制的研究有限，特别是短链全氟辛酸。在这项研究中，我们研究了PFAAs与鱼血清蛋白的结合亲和力和全氟烷基羧酸盐（C7-C11 PFCAs）和全氟烷基磺酸盐（C4，C6和C8 PFSAs）在鲤鱼（Cyprinus carpio）中的组织分布，包括PFOS和全氟己烷磺酸盐（PFHxS）的异构体。对于PFCAs和PFSAs，鱼血清蛋白结合常数（KA）和生物富集因子（BCF）随着碳链长度的增加而增加。PFHxS（C6 PFSA）的KA值远高于C7-C11 PFCAs，而BCF值远低于C7-C11 PFCAs。这表明，不仅鱼血蛋白，而且肝脏和肾脏中的其他蛋白质也介导了PFAAs在鱼体内的积累。PFHxS在肝脏与血液、肾脏与血液中的最低浓度比表明，PFHxS不能通过脂肪酸结合蛋白和有机阴离子转运体有效转运至肝脏和肾脏。全氟辛烷磺酸和全氟硫化氢显示出不同的消除途径，尽管其线性（正）异构体在鱼类体内的累积量大于相应的分支（br-）异构体。之后，-PFOS更多地通过粪便消除，而-PFOS更多地通过尿液消除;而PFHxS异构体则观察到相反的趋势。
With regulations on the manufacture and usage of perfluorooctanoate (PFOA), perfluorooctane sulfonate (PFOS) and related compounds, short-chain perfluoroalkyl acids (PFAAs) are increasingly being used as alternatives. However, there are limited studies on their bioaccumulation mechanisms, especially for short-chain PFAAs. In this study, we examined the binding affinity of PFAAs with fish serum proteins and tissue distributions of perfluoroalkyl carboxylates (C7-C11 PFCAs) and perfluoroalkyl sulfonates (C4, C6, and C8 PFSAs) in carp (Cyprinus carpio), including the isomers of PFOS and perfluorohexane sulfonate (PFHxS). For both PFCAs and PFSAs, the fish serum protein binding constant (KA) and bioconcentration factor (BCF) increased with an increase in the carbon chain length. PFHxS (C6 PFSA) had a much higherKAbut displayed a much lower BCF than those of C7-C11 PFCAs. It indicated that not only fish blood proteins, but also other proteins in the liver and kidney, mediated the accumulation of PFAAs in fish. The lowest concentration ratios of PFHxS in liver to blood and in kidney to blood suggested that it could not be effectively transported to liver and kidney by fatty acid binding proteins and organic anion transporters. PFOS and PFHxS displayed different elimination pathways, although their linear (n-) isomers were accumulated more in fish than the corresponding branched (br-) isomers. Then-PFOS was eliminated more via the feces butbr-PFOS was eliminated more via the urine; while the opposite trend was observed for PFHxS isomers.