Transformation and expression of a stilbene synthase gene of Vitis vinifera L. in barley and wheat for increased fungal resistance
Transformation and expression of a stilbene synthase gene of Vitis vinifera L. in barley and wheat for increased fungal resistance
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DOI：
10.1007/s001220050832
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发表时间：
1998-06-01
影响因子：
5.4
通讯作者：
Lörz, H
中科院分区：
文献类型：
--
作者：
Leckband, G;Lörz, H
用葡萄Vst1基因(Vst1基因)进行基因枪法转化大麦和小麦，可使外源植物保卫素白藜芦醇在转化植株中表达。以小孢子为外植体再生转基因大麦植株，并在BASTA上筛选到转基因小麦幼胚植株。对转基因大麦、小麦及其后代的基因组进行了Southern-Blot分析，结果表明转基因大麦、小麦及其后代基因组中稳定整合了该基因。在T-1和T-2子代植株中证明了二苯乙烯合成酶启动子的诱导和创伤和侵染诱导后二苯乙烯合成酶特异性mRNA的瞬时表达。利用花椰菜花叶病毒35S(CaMV 35S)启动子的增强子序列，观察到Vst1基因在二苯乙烯合成酶启动子控制下的表达增强。对T1代植株的二苯乙烯合成酶活性进行了分析。初步的病理结果表明，转基因大麦植株对灰霉病的抗性增强，作为模式实验系统。
Transformation of barley and wheat via particle bombardment with a gene derived from Vitis vinifera L. (Vst1 gene) resulted in the expression of the foreign phytoalexin, resveratrol, in the transformed plants. Transgenic barley plants were regenerated from microspores and transgenic wheat plants from immature embryos were both selected on Basta. Stable integration of the gene in the genomes of transgenic barley and wheat plants, as well as their progeny, was analysed by Southern-blot analysis. The induction of the stilbene synthase promoter and the transient expression of stilbene synthase-specific mRNA after induction by wounding and infection were proofed in T-1 and T-2 progeny plants. An enhanced expression of the Vst1 gene under control of the stilbene synthase promoter was observed with enhancer sequences from the cauliflower mosaic virus 35s (CaMV 35s) promoter. The enzyme activity of the stilbene synthase was analysed in T1 progeny plants. The first pathological results indicated an increased resistance of transgenic barley plants to Botrytis cinerea used as a model experimental system.