Unveiling the Identity of Wenwan Walnuts and Phylogenetic Relationships of Asian Juglans Species Using Restriction Site-Associated DNA-Sequencing.
Unveiling the Identity of Wenwan Walnuts and Phylogenetic Relationships of Asian Juglans Species Using Restriction Site-Associated DNA-Sequencing.
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利用限制性位点相关 DNA 测序揭示文湾核桃的身份和亚洲核桃物种的系统发育关系
DOI：
10.3389/fpls.2017.01708
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发表时间：
2017
影响因子：
5.6
通讯作者：
Lin QW
中科院分区：
文献类型：
--
作者：
Mu XY;Sun M;Yang PF;Lin QW
核桃属植物在世界范围内具有重要的生态和经济价值。在中国，文玩核桃被贵族和贵族收藏已有2000多年的历史。作为亚洲核桃的多样性中心，我国有5种核桃广泛分布。其中最著名的是Mahetao（J. hopeiensis），这是一个没有特征的物种，主要是栽培。河北野核桃是河北省特有的珍稀树种。由于以前使用的分子标记和叶绿体和核基因组之间的异质性的变化很小，确定核桃属物种之间的系统发育关系一直具有挑战性，并阻碍了随后的进化推论。在这项研究中，我们收集了足够的材料，栽培和野生的核桃构建良好的解决亚洲核桃物种的系统发育树。我们使用高通量全基因组限制性位点相关DNA测序方法。因此，J. hopeiensis的身份已得到明确解决。本研究结果表明，河北核桃是核桃和东北核桃的杂种。而J. hopeiensis、J. regia和J. sigillata应视为核桃组的一个种。此外，根据形态学和分子生物学的研究，臭椿、山核桃和东北核桃可能代表一个种。这些结果得到了种群结构分析和形态学比较的支持。我们建议，生长在野外的J. hopeiensis树木应该保护，因为他们的坚果的经济价值。这些树木对贫困社区可能特别重要。此外，它们可以作为与提高食用核桃产量相关的宝贵遗传资源。2b-RAD方法是一个可行的选择，为未来的系统发育研究的核桃属物种以及其他植物物种。
Juglans species have considerable ecological and economic value worldwide. In China, Wenwan walnuts have been collected by aristocrats and noblemen for more than 2000 years. As a diversity center of Asian Juglans, five species are widely distributed in China. The most famous of these is Mahetao (J. hopeiensis), which is an uncharacterized species that is mostly cultivated. Wild J. hopeiensis individuals are very rare and are endemic to Hebei Province. Because of the minimal variations in previously used molecular markers and the heterogeneity between chloroplast and nuclear genomes, determining the phylogenetic relationships among the Juglans species has been challenging, and has hindered subsequent evolutionary inferences. In this study, we collected enough materials for both cultivated and wild Mahetao to construct well-resolved phylogenetic trees for Asian Juglans species. We used a high-throughput genome-wide restriction site-associated DNA sequencing method. Consequently, the identity of J. hopeiensis has been clearly resolved. Our results indicate that J. hopeiensis is a hybrid of J. regia and J. mandshurica. However, J. hopeiensis, J. regia and J. sigillata should be considered as a single species from section Juglans. Additionally, J. ailantifolia, J. cathayensis, and J. mandshurica likely represent one species from section Cardiocaryon according to morphological and molecular studies. These results are supported by population structure analysis and morphological comparison. We propose that J. hopeiensis trees growing in the wild should be conserved because of the economic value of their nuts. These trees may be of particular importance to impoverished communities. Furthermore, they may serve as a valuable genetic resource relevant for enhancing the production of edible walnuts. The 2b-RAD method is a viable option for future phylogenetic studies of Juglans species as well as other plant species.
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影响因子：
6.5
作者：
Leaché AD;Banbury BL;Felsenstein J;de Oca AN;Stamatakis A
通讯作者：
Stamatakis A
RADtyping：用于在人群作图中准确进行 De Novo 共显性和显性 RAD 基因分型的集成包
影响因子：
3.7
作者：
Fu X;Dou J;Mao J;Su H;Jiao W;Zhang L;Hu X;Huang X;Wang S;Bao Z
通讯作者：
Bao Z
影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
2.8
作者：
Ciarmiello, Loredana F.;Piccirillo, Pasquale;Woodrow, Pasqualina
通讯作者：
Woodrow, Pasqualina
影响因子：
3.7
作者：
Hipp AL;Eaton DA;Cavender-Bares J;Fitzek E;Nipper R;Manos PS
通讯作者：
Manos PS