Study of elastic fiber organization by scanning force microscopy
Study of elastic fiber organization by scanning force microscopy
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DOI：
10.1016/s0945-053x(98)90126-3
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发表时间：
1998-04-01
期刊：
影响因子：
6.9
通讯作者：
Valdrè, U
中科院分区：
文献类型：
--
作者：
Ronchetti, IP;Alessandrini, A;Valdrè, U
通过原子力显微镜观察牛项韧带的弹性纤维，并将数据与常规和冷冻断裂电子显微镜获得的数据进行比较。对新鲜分离的弹性蛋白纤维以及韧带片段的薄片进行分析，这些片段在松弛或拉伸条件下固定和嵌入。结果证实，至少在牛项韧带中，弹性纤维由珠状细丝组成，可以通过沿所施加的力的方向拉伸来定向。此外，原子力显微镜显示，这些珠状细丝通过周期性桥横向连接，在拉伸时变得更加明显。数据清楚地表明，弹性蛋白分子至少在超分子水平上以相当有序的阵列组织，并且尝试描述牛颈韧带中的弹性蛋白组织。
Elastic fibers of beef ligamentum nuchae were observed by atomic force microscopy and data compared with those obtained by conventional and freeze-fracture electron microscopy. Fresh isolated elastin fibers as well as thin sections of ligament fragments, which were fixed and embedded either in relaxed or in stretched, conditions, were analysed. The results confirm that, at least in beef ligamentum nuchae, elastic fibers consist of beaded filaments which can be oriented by stretching in the direction of the force applied. Moreover, atomic force microscopy revealed that these beaded filaments are laterally connected by periodical bridges which become more pronounced upon stretching. The data clearly show that elastin molecules are organized in a rather ordered array, at least at the super-molecular level, and a depiction of the elastin organization in beef ligament um nuchae is attempted.