A Comparison of the Process of Remodeling of Hydroxyapatite/Poly-D/L-Lactide and Beta-Tricalcium Phosphate in a Loading Site.
A Comparison of the Process of Remodeling of Hydroxyapatite/Poly-D/L-Lactide and Beta-Tricalcium Phosphate in a Loading Site.
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DOI：
10.1155/2015/730105
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发表时间：
2015
影响因子：
--
通讯作者：
Hara Y
中科院分区：
文献类型：
--
作者：
Akagi H;Ochi H;Soeta S;Kanno N;Yoshihara M;Okazaki K;Yogo T;Harada Y;Amasaki H;Hara Y
目前，最常用的生物可吸收支架是由β-磷酸三钙(β-TCP)制成的，由羟基磷灰石(HA)和聚-D/L-丙交酯(PDLLA)混合而成的支架有望成为一种新型的生物可吸收支架。本研究的目的是评估HA/PDLLA支架与β-Tcp支架在加载现场的实用性。狗接受了手术，用可生物吸收的支架替换了一段胫骨。随访期结束后，对支架进行组织学分析。与β-Tcp支架相比，HA/PDLLA支架显示出相似的骨形成和更好的细胞和组织渗透，如Villanueva Goldner染色所示。此外，银染和Von Willebrand因子和组织蛋白酶K免疫组织化学染色显示HA/PDLLA支架内有较好的细胞浸润。已渗透到HA/PDLLA支架中的纤维组织和细胞分别检测出I型胶原和RUNX2阳性，表明已渗透到HA/PDLLA支架中的组织和细胞具有向骨分化的潜能。因此，HA/PDLLA支架作为一种新型的生物可吸收支架有望在临床上得到应用。
Currently, the most commonly used bioresorbable scaffold is made of beta-tricalcium phosphate (β-TCP); it is hoped that scaffolds made of a mixture of hydroxyapatite (HA) and poly-D/L-lactide (PDLLA) will be able to act as novel bioresorbable scaffolds. The aim of this study was to evaluate the utility of a HA/PDLLA scaffold compared to β-TCP, at a loading site. Dogs underwent surgery to replace a section of tibial bone with a bioresorbable scaffold. After the follow-up period, the scaffold was subjected to histological analysis. The HA/PDLLA scaffold showed similar bone formation and superior cell and tissue infiltration compared to the β-TCP scaffold, as seen after Villanueva Goldner staining. Moreover, silver staining and immunohistochemistry for Von Willebrand factor and cathepsin K demonstrated better cell infiltration in the HA/PDLLA scaffold. The fibrous tissue and cells that had infiltrated into the HA/PDLLA scaffold tested positive for collagen type I and RUNX2, respectively, indicating that the tissue and cells that had infiltrated into the HA/PDLLA scaffold had the potential to differentiate into bone. The HA/PDLLA scaffold is therefore likely to find clinical application as a new bioresorbable scaffold.