Stacking and simultaneous determination of estrogens in water samples by CE with electrochemical detection.
Stacking and simultaneous determination of estrogens in water samples by CE with electrochemical detection.
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DOI：
10.1002/jssc.201200412
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发表时间：
2013
影响因子：
3.1
通讯作者：
Ping Li;Wenjie Zhao;Xiaoning Wang;G. Shi;Tianshu Zhou
中科院分区：
文献类型：
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作者：
Ping Li;Wenjie Zhao;Xiaoning Wang;G. Shi;Tianshu Zhou
建立了一种快速、灵敏的同时测定雌酮、17β-雌二醇和雌三醇的方法。考察了影响分离和检测的几个重要因素。在最佳条件下，在0.06 mol/L氢氧化钠溶液中，雌激素在14 min内即可分离。在0.5%NaCl溶液中，三种雌激素在0.2 ~ 10 μmol/L范围内线性关系良好，相关系数大于0.9993。雌三醇、17β-雌二醇和雌酮的检出限分别为8.9 × 10 - 8、6.7 × 10 - 8和1.1 × 10 - 7 mol/L（S/N = 3）。该方法用于自来水厂、自来水、鱼塘、河水等水样的分析，回收率在90.8- 108.9%之间，相对标准偏差（RSD）<4.69%。结果表明，该方法制备简便、灵敏度高、重现性好，可用于环境水样的快速测定。
A rapid and sensitive method based on transient ITP and field enhancement in CE with electrochemical detection at copper disk electrode was developed for the simultaneous separation and determination of three estrogens: estrone, 17β-estradiol, and estriol. The effects of several important factors that influence the separation and detection were investigated. Under the optimum conditions, the estrogens could be separated in 0.06 mol/L sodium hydroxide solution within 14 min. With transient ITP by addition of 0.5% NaCl, a good linear response was obtained for three estrogens from 0.2 to 10 μmol/L, with correlation coefficients higher than 0.9993. The detection limits were 8.9 × 10(-8) , 6.7 × 10(-8) , and 1.1 × 10(-7) mol/L (S/N = 3) for estriol, 17β-estradiol, and estrone, respectively. This method was successfully employed to analyze different water samples from waterworks, tap water, fishpond, and river samples with recoveries in the range of 90.8-108.9%, and RSDs < 4.69%. The satisfied results demonstrated that this method was of convenient preparation, high sensitivity, and good repeatability, which could be applied to the rapid determination of environmental water samples.