Closely Related NAC Transcription Factors of Tomato Differentially Regulate Stomatal Closure and Reopening during Pathogen Attack
Closely Related NAC Transcription Factors of Tomato Differentially Regulate Stomatal Closure and Reopening during Pathogen Attack
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DOI：
10.1105/tpc.114.128272
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发表时间：
2014-07-01
期刊：
影响因子：
11.6
通讯作者：
Li, Chuanyou
中科院分区：
文献类型：
--
作者：
Du, Minmin;Zhai, Qingzhe;Li, Chuanyou
为了限制病原体的进入，植物关闭气孔作为先天免疫的组成部分。为了对抗这种防御，紫丁香假单胞菌番茄产生冠状物质(COR)，它模仿茉莉酸(JA)，重新打开气孔，以防止细菌进入。人们认为脱落酸(ABA)在调节细菌触发的气孔关闭中起着核心作用，气孔重新开放需要JA/COR途径，但下游的信号事件尚不清楚。我们研究了番茄(Solanum Lycopsicum)的气孔免疫，报道了两个同源NAC(针对NAM、ATAF1、2和CUC2)转录因子JA2(针对茉莉酸2)和JA2L(针对类似JA2)在调节病原菌触发的气孔运动中的不同作用。ABA激活了JA2的表达，对JA2的遗传操作揭示了其在ABA介导的气孔关闭中的积极作用。我们证明JA2通过调节ABA生物合成基因的表达来发挥这种作用。相反，JA和COR激活了JA2L的表达，并且JA2L的遗传操作显示了其在JA/COR介导的气孔重新开放中的积极作用。我们证明JA2L通过调节参与水杨酸代谢的基因的表达来执行这一作用。因此，这些密切相关的NAC蛋白通过不同的机制对病原菌诱导的气孔关闭和重新开放进行不同的调节。
To restrict pathogen entry, plants close stomata as an integral part of innate immunity. To counteract this defense, Pseudomonas syringae pv tomato produces coronatine (COR), which mimics jasmonic acid (JA), to reopen stomata for bacterial entry. It is believed that abscisic acid (ABA) plays a central role in regulating bacteria-triggered stomatal closure and that stomatal reopening requires the JA/COR pathway, but the downstream signaling events remain unclear. We studied the stomatal immunity of tomato (Solanum lycopersicum) and report here the distinct roles of two homologous NAC (for NAM, ATAF1,2, and CUC2) transcription factors, JA2 (for jasmonic acid2) and JA2L (for JA2-like), in regulating pathogen-triggered stomatal movement. ABA activates JA2 expression, and genetic manipulation of JA2 revealed its positive role in ABA-mediated stomatal closure. We show that JA2 exerts this effect by regulating the expression of an ABA biosynthetic gene. By contrast, JA and COR activate JA2L expression, and genetic manipulation of JA2L revealed its positive role in JA/COR-mediated stomatal reopening. We show that JA2L executes this effect by regulating the expression of genes involved in the metabolism of salicylic acid. Thus, these closely related NAC proteins differentially regulate pathogen-induced stomatal closure and reopening through distinct mechanisms.