Parsley extract inhibits in vitro and ex vivo platelet aggregation and prolongs bleeding time in rats
Parsley extract inhibits in vitro and ex vivo platelet aggregation and prolongs bleeding time in rats
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DOI：
10.1016/j.jep.2009.05.014
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发表时间：
2009-08-17
影响因子：
5.4
通讯作者：
Mekhfi, Hassane
中科院分区：
文献类型：
--
作者：
Gadi, Dounia;Bnouham, Mohamed;Mekhfi, Hassane
许多心血管疾病与血小板活性增加有关。在摩洛哥，欧芹（欧芹，伞形科）是用于治疗心血管疾病，如动脉高血压的草药之一。在这项研究中，粗水提取物（CAE）的欧芹进行了评价其抗血小板活性在实验动物的血小板聚集在体外和离体，并在体内出血时间。用CAE预孵育血小板后，监测体外聚集。在口服治疗后进行出血时间和离体聚集。CAE呈剂量依赖性地抑制凝血酶、ADP、胶原和肾上腺素诱导的血小板聚集。口服CAE（3g/kg）可显著抑制离体血小板聚集（p < 0.001），延长出血时间（p < 0.001），而血小板量无明显变化。CAE的出血时间延长可能归因于观察到的血小板聚集抑制。这些影响可能部分与提取物中存在的多酚化合物有关。这些结果支持了以下假设：欧芹的膳食摄入可能有利于血小板过度活化的正常化，有利于心血管疾病的营养预防，并可能对开发新的预防策略产生潜在的影响。(C)2009年由Elsevier爱尔兰有限公司出版。
Many cardiovascular diseases are associated with an increase in blood platelet activity. In Morocco, parsley (Petroselinum crispum, Apiaceae) is one of the medicinal herbs used to treat cardiovascular diseases such as arterial hypertension. In this study, crude aqueous extract (CAE) of parsley was evaluated for its anti-platelet activity in experimental animals on platelet aggregation in vitro and ex vivo; and on bleeding time in vivo. The in vitro aggregation was monitored after pre-incubation of platelets with CAE. The bleeding time and ex vivo aggregation were performed after oral treatment. CAE inhibited dose dependently platelet aggregation in vitro induced by thrombin, ADP, collagen and epinephrine. The oral administration of CAE (3 g/kg) inhibited significantly (p < 0.001) platelet aggregation ex vivo and prolonged bleeding time (p < 0.001) without changes in the platelet amount. The prolongation of bleeding time by CAE may be attributed to the observed inhibition of platelet aggregation. These effects could be related in part to the polyphenolic compounds present in the extract. These results support the hypothesis that the dietary intake of parsley may be benefit in the normalization of platelet hyperactivation, in the nutritional prevention of cardiovascular diseases and are potentially interesting in the development of new prevention strategies. (C) 2009 Published by Elsevier Ireland Ltd.