Genome-wide identification of cotton GRAM family proteins reveals that GRAM31 regulates fiber length
Genome-wide identification of cotton GRAM family proteins reveals that GRAM31 regulates fiber length
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棉花 GRAM 家族蛋白的全基因组鉴定揭示 GRAM31 调节纤维长度
DOI：
10.1093/jxb/eraa597
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发表时间：
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影响因子：
6.9
通讯作者：
Lili Tu
中科院分区：
文献类型：
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作者：
Zhengxiu Ye;Lu Qiao;Xiangyin Luo;Xinyuan Chen;Xianlong Zhang;Lili Tu
葡萄糖基转移酶、Rab-like GT3激活剂和肌微管蛋白（myotubularins，MTR）结构域在真核细胞中高度保守，并且存在于参与膜相关过程的蛋白质中。在棉花中还没有功能上的特征。在这项研究中，我们确定了164个基因编码的结构域蛋白在四个棉花物种，包括两个亚科。结果表明，GhGRAM 31基因在陆地棉纤维发育的快速伸长期表达，可能控制纤维长度; GhGRAM 31-RNAi转基因棉纤维伸长受到抑制，成熟纤维变短，这与纤维发育相关基因的表达变化有关。此外，在RNAi株系中，衣分和种子大小也降低。进一步的研究表明，GhGRAM 31直接与另外两种GRAM结构域蛋白GhGRAM 5和GhGRAM 35相互作用。GhGRAM 5也与转录因子GhTTG 1相互作用，而GhGRAM 35与转录因子GhHOX 1和GhHD 1相互作用。GhGRAM 31和GhGRAM 35的共表达能够促进棉花原生质体中GhHD 1的转录活性。我们的研究结果为棉花GRAM结构域蛋白家族的生物学功能提供了新的见解，所选基因具有在未来纤维遗传改良计划中利用的潜力。
The glucosyltransferases, Rab-like GTPase activators and myotubularins (GRAM) domain is highly conserved in eukaryotic cells and is found in proteins involved in membrane-associated processes. GRAM domain proteins have not yet been functionally characterized in cotton. In this study, we identified 164 genes encoding GRAM domain proteins in four cotton species, comprising two subfamilies. InGossypium hirsutum, our transcriptome data showed thatGhGRAM31was predominantly expressed during the rapid elongation stage of fiber development and that it might control fiber length.GhGRAM31-RNAi transgenic cotton lines showed inhibition of fiber elongation and produced shorter mature fibers, and this was coupled with expression changes of genes related to fiber development. In addition, lint percentage and seed size were also decreased in the RNAi lines. Further examination revealed that GhGRAM31 directly interacts with two other GRAM-domain proteins, GhGRAM5 and GhGRAM35. GhGRAM5 also interacts with the transcription factor GhTTG1, while GhGRAM35 interacts with the transcription factors GhHOX1 and GhHD1. Co-expression of GhGRAM31 and GhGRAM35 was able to promote GhHD1 transcription activity in cotton protoplasts. Our results provide new insights into the biological function of the GRAM-domain protein family in cotton, and selected genes have the potential to be utilized in future programs for the genetic improvement of fibers.