S‐Phase cells in diseased human liver determined by an in Vitro BrdU‐anti‐BrdU method
S‐Phase cells in diseased human liver determined by an in Vitro BrdU‐anti‐BrdU method
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通过体外 BrdU-抗 BrdU 方法测定患病人肝脏中的 S 期细胞
DOI：
10.1002/hep.1840080611
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发表时间：
1988
期刊：
影响因子：
13.5
通讯作者：
T. Ono
中科院分区：
文献类型：
--
作者：
A. Shimizu;K. Tarao;S. Takemiya;Masaoki Harada;Tohru Inoue;T. Ono
用溴脱氧尿苷单克隆抗体，我们研究了人肝癌，肝硬化，慢性活动性肝炎和酒精性肝纤维化的细胞动力学。通过活检或手术采集标本，并立即与0.1%溴脱氧尿苷溶液在37°C下孵育45 min。体外标记后，使用抗溴脱氧尿苷单克隆抗体作为第一抗体，通过抗生物素蛋白生物素-过氧化物酶复合物法测定S期细胞核摄取的溴脱氧尿苷。计数1，000个肝细胞中阳性细胞核的数量，并以每千个细胞中的溴脱氧尿苷标记指数表示。
Using a monoclonal antibody to bromodeoxyuridine, we studied the cell kinetics of human hepatocellular carcinoma, liver cirrhosis, chronic active hepatitis and alcoholic liver fibrosis. Specimens were taken either by biopsy or surgery and immediately incubated with 0.1% bromodeoxyuridine solution at 37°C for 45 min. After in vitro labeling, the bromodeoxyuridine taken up by the nuclei of S‐phase cells was determined by the avidinbiotin‐peroxidase complex method, using an anti‐bromodeoxyuridine monoclonal antibody as the first antibody. The number of positive nuclei in 1,000 hepatic cells was counted, and the bromodeoxyuridine labeling index was expressed per thousand.