An antisense promoter of the murine c-myc gene is localized within intron 2.
An antisense promoter of the murine c-myc gene is localized within intron 2.
复制标题
鼠 c-myc 基因的反义启动子位于内含子 2 内。
DOI：
10.1128/mcb.12.3.1324-1329.1992
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发表时间：
1992
影响因子：
5.3
通讯作者：
Sonenshein,GE
中科院分区：
文献类型：
--
作者：
Spicer,DB;Sonenshein,GE
Previously we have demonstrated the existence of stable transcripts from the noncoding strand of a rearranged c-mycgene in murine plasmacytomas in which the oncogene has translocated to an immunoglobulin constant-region gene element (M. Dean, R. B. Kent, and G. E. Sonenshein, Nature [London] 305:443-446, 1983). The resulting RNAs are chimeric, containing c-mycantisense and immunoglobulin sense sequences. A normal unrearranged murine c-mycgene is transcribed in the antisense orientation throughout much of the gene; however, stable transcripts have not been detected. In this study, using Northern (RNA) blot, S1 nuclease, and primer extension analyses, we have mapped the 5'end of the stable chimeric transcripts to a site 175 bp from the start of exon 3, within intron 2 of the c-mycgene. In vitro transcription assays with constructs containing this site and 400 bp upstream, in the antisense orientation, and nuclear extracts from plasmacytoma cells, as well as a number of cell lines with normal unrearranged c-mycgenes, indicated that this promoter was functional. This finding was confirmed in transient transfection assays using the antisense promoter linked to the chloramphenicol acetyltransferase reporter gene. These results suggest that a normal promoter of antisense transcription is used following c-mycgene translocation.
