Functional interaction between the homeotic genes fbp1 and pMADS1 during petunia floral organogenesis.
Functional interaction between the homeotic genes fbp1 and pMADS1 during petunia floral organogenesis.
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DOI：
10.1105/tpc.7.5.507
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发表时间：
1995-05
期刊：
影响因子：
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通讯作者：
G. Angenent;M. Busscher;J. Franken;H. Dons;A. V. van Tunen
中科院分区：
文献类型：
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作者：
G. Angenent;M. Busscher;J. Franken;H. Dons;A. V. van Tunen
矮牵牛MADS盒花结合蛋白（fbp）基因1代表决定第二和第三花轮器官的身份的B类同源异型基因。与金鱼草属基因globosa和拟南芥属基因pistillata高度同源的fbp1的抑制导致花瓣转化为萼片，雄蕊转化为心皮。与fbp 1相反，矮牵牛同源异型基因pMADS 1编码与金鱼草蛋白DEFICIENS同源的蛋白质，已被证明仅参与花瓣的形成。我们证明，诱导fbp1的建立独立的pMADS1，而在后期的发展阶段，fbp1的上调pMADS1在花瓣中。另一方面，pMADS 1表达的诱导和维持不受fbp 1的影响。为了获得fbp 1和pMADS 1之间的功能相互作用的信息，fbp 1共抑制突变体与轻度表型改变的绿色花瓣突变体，其中pMADS 1的表达被废除。后代植物，杂合的pMADS1基因，有一个更明显的逆转从花瓣到萼片比观察到的亲本fbp1突变体的花。第3轮器官的形态无明显变化。这些观察结果，连同突变体花中pMADS1和fbp1的表达水平，为体内PMADS1对fbp1的功能控制提供了证据。
The petunia MADS box floral binding protein (fbp) gene 1 represents a class B homeotic gene determining the identity of second and third floral whorl organs. Suppression of fbp1, which is highly homologous to the Antirrhinum gene globosa and Arabidopsis gene pistillata, results in the conversion of petals to sepals and stamens to carpels. In contrast to fbp1, the petunia homeotic gene pMADS1, encoding a protein homologous to the Antirrhinum protein DEFICIENS, has been shown to be involved in the formation of petals only. We demonstrated that the induction of fbp1 is established independent of pMADS1, whereas at later developmental stages, fbp1 is up-regulated by pMADS1 in petals. On the other hand, the induction and maintenance of pMADS1 expression are not affected by fbp1. To obtain information about the functional interaction between fbp1 and pMADS1, an fbp1 cosuppression mutant with mild phenotypic alterations was crossed with a green petals mutant in which pMADS1 expression was abolished. Progeny plants, heterozygous for the pMADS1 gene, had flowers with a more pronounced reversion from petals into sepals than was observed for the parent fbp1 mutant. The morphology of the third whorl organs was not changed. These observations, together with expression levels of pMADS1 and fbp1 in mutant flowers, provide evidence for functional control of fbp1 by PMADS1 in vivo.