Engineering Advanced Capsosomes: Maximizing the Number of Subcompartments, Cargo Retention, and Temperature-Triggered Reaction
Engineering Advanced Capsosomes: Maximizing the Number of Subcompartments, Cargo Retention, and Temperature-Triggered Reaction
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DOI：
10.1021/nn901843j
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发表时间：
2010-03-01
期刊：
影响因子：
17.1
通讯作者：
Caruso, Frank
中科院分区：
文献类型：
--
作者：
Chandrawati, Rona;Hosta-Rigau, Leticia;Caruso, Frank
先进的细胞模拟需要大量但可控的小室，包裹在支架内，配备触发装置来启动、终止和潜在地重新启动酶反应。最近推出的胶囊，即含有数千种脂质体的聚合物胶囊，是创造人工细胞的一个有前途的平台。胶囊体是通过将脂质体和聚合物顺序分层到颗粒模板上，然后去除模板核心而形成的。在这里，我们设计了先进的胶囊，并展示了控制小舱数量和货物装载程度的能力。为了实现这一点，我们使用了一系列的聚合物分离层和脂质体来形成由多层酶负载脂质体组成的功能性胶囊。酶分析转化率的差异被用来验证多层完整的酶载脂质体组装在聚合物水凝胶胶囊中。在胶囊组装过程中，脂质体亚室中包裹的货物的大小依赖的保留以及它对环境pH变化的依赖也被研究。我们进一步表明，温度可以用来在脂质体亚室的相变温度触发酶反应，并且包裹的酶可以在随后的几次转化中重复利用。这些具有定制特性的工程化胶囊为功能人工细胞的开发提供了新的机会。
Advanced mimics of cells require a large yet controllable number of subcompartments encapsulated within a scaffold, equipped with a trigger to initiate, terminate, and potentially restart an enzymatic reaction. Recently introduced capsosomes, polymer capsules containing thousands of liposomes, are a promising platform for the creation of artificial cells. Capsosomes are formed by sequentially layering liposomes and polymers onto particle templates, followed by removal of the template cores. Herein, we engineer advanced capsosomes and demonstrate the ability to control the number of subcompartments and hence the degree of cargo loading. To achieve this, we employ a range of polymer separation layers and liposomes to form functional capsosomes comprising multiple layers of enzyme-loaded liposomes. Differences in conversion rates of an enzymatic assay are used to verify that multilayers of intact enzyme-loaded liposomes are assembled within a polymer hydrogel capsule. The size-dependent retention of the cargo encapsulated within the liposomal subcompartments during capsosome assembly and its dependence on environmental pH changes are also examined. We further show that temperature can be used to trigger an enzymatic reaction at the phase transition temperature of the liposomal subcompartments, and that the encapsulated enzymes can be utilized repeatedly in several subsequent conversions. These engineered capsosomes with tailored properties present new opportunities en route to the development of functional artificial cells.