MOLECULAR-BASIS OF FUNCTIONAL DIVERSITY OF VOLTAGE-GATED POTASSIUM CHANNELS IN MAMMALIAN BRAIN
MOLECULAR-BASIS OF FUNCTIONAL DIVERSITY OF VOLTAGE-GATED POTASSIUM CHANNELS IN MAMMALIAN BRAIN
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DOI：
10.1002/j.1460-2075.1989.tb08483.x
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发表时间：
1989-11-01
期刊：
影响因子：
11.4
通讯作者：
PONGS, O
中科院分区：
文献类型：
--
作者：
STUHMER, W;RUPPERSBERG, JP;PONGS, O
克隆和测序发现，一个基因家族编码多个高度同源的K+通道形成蛋白(RCK)。显微注射体外转录的RCK特异性RNA后非洲爪哇卵母细胞表达的通道的功能鉴定表明，每种RCK蛋白形成的K+通道在功能和药理特性上都有很大的不同。这表明哺乳动物脑中电压门控性K+通道多样性的分子基础至少部分是基于一个基因家族对几种RCK蛋白的表达，以及它们组装成具有不同功能特性的同源寡聚体K+通道。
Cloning and sequencing of cDNAs isolated from a rat cortex cDNA library reveals that a gene family encodes several highly homologous K+ channel forming (RCK) proteins. Functional characterization of the channels expressed in Xenopus laevis oocytes following microinjection of in vitro transcribed RCK‐specific RNAs shows that each of the RCK proteins forms K+ channels that differ greatly in both their functional and pharmacological properties. This suggests that the molecular basis for the diversity of voltage‐gated K+ channels in mammalian brain is based, at least partly, on the expression of several RCK proteins by a family of genes and their assembly to homooligomeric K+ channels with different functional properties.