Catalytic mechanisms of restriction and homing endonucleases
Catalytic mechanisms of restriction and homing endonucleases
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DOI：
10.1021/bi020467h
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发表时间：
2002-11-26
期刊：
影响因子：
2.9
通讯作者：
Stoddard, BL
中科院分区：
文献类型：
--
作者：
Galburt, EA;Stoddard, BL
对Ⅱ型限制性内切酶和归巢酶的催化机理进行了讨论和比较。简要回顾了磷酰基转移的化学和典型的单金属和双金属核酸内切酶的机制提供了沿着可能的未来方向在研究核酸内切酶的活性位点。II型限制性内切核酸酶的讨论包括对典型活性位点结构基序的一般结构的描述，随后是单金属和双金属机制的更深入的例子。归巢核酸内切酶部分由四个部分组成，描述了关于四个I组内含子归巢核酸内切酶家族的切割机制已知的内容：LAGLIDADG、His-Cys盒、H-N-H和GIY-YIG。
The catalytic mechanisms of type II restriction endonucleases and homing endonucleases are discussed and compared. Brief reviews of the chemistry of phosphoryl transfers and canonical one-metal and two-metal endonucleolytic mechanisms are provided along with possible future directions in the study of endonuclease active sites. The discussion of type II restriction endonucleases is comprised of a description of the general architecture of the canonical active site structural motif followed by more in-depth examples of one- and two-metal mechanisms. The homing endonuclease section is comprised of four sections describing what is known regarding the cleavage mechanisms of the four group I intron homing endonuclease families: LAGLIDADG, His-Cys box, H-N-H, and GIY-YIG.