Surface modification of hydroxyapatite nanocrystallite by a small amount of terbium provides a biocompatible fluorescent probe
Surface modification of hydroxyapatite nanocrystallite by a small amount of terbium provides a biocompatible fluorescent probe
复制标题
少量铽对羟基磷灰石纳米晶进行表面修饰，提供生物相容性荧光探针
DOI：
10.1021/jp8026463
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发表时间：
2008-08-14
影响因子：
3.7
通讯作者：
Tang, Ruikang
中科院分区：
文献类型：
--
作者：
Li, Ling;Liu, Yukan;Tang, Ruikang
羟基磷灰石（HAP）作为生物骨和牙釉质的主要无机成分，具有良好的生物相容性和生物活性。我们最近的工作表明，20 nm的HAP可以很容易地被活细胞内化。然而，HAP纳米颗粒不发光，使得它们在体外实验期间难以在活细胞中测量。提出了一种新型的铽掺杂20 nm羟基磷灰石的无机生物探针。HAP颗粒表面的钙离子可以部分被Tb取代，这导致20 nm的Tb-HAP颗粒。掺杂Tb的HAP变得发光，并且可以在488 nm的可见光激发下观察到。与常用的探针相比，修饰后的纳米HAP具有更好的光稳定性，且几乎无毒。重要的是，HAP的特性保持后，用少量的Tb的表面改性。与培养的兔骨髓间充质干细胞（MSCs）共同孵育后，在荧光显微镜下可以清楚地观察到活细胞中内化的HAP的发光。透射电子显微镜分析也证实了MSC对颗粒的摄取。结果表明，修饰后的HAP是一种稳定的细胞生物探针。我们认为，Tb掺杂的HAP应该有很大的潜力来跟踪纳米粒子的演变，这是纳米生物技术的一个关键课题。
As the main inorganic component of biological bone and tooth enamel, hydroxyapatite (HAP) is highly biocompatible and bioactive. Our recent work has shown that 20 nm HAP can be readily internalized by living cells. However, HAP nanoparticles are not luminescent so that they are difficult to measure in living cells during in vitro experiments. A novel inorganic biological probe was suggested by doping 20 nm HAP with terbium. The calcium ions on the HAP particle surface could be partially replaced by Tb, which resulted in the 20 nm Tb-HAP particles. The Tb-doped HAP became luminescent and could be observed under a visible excitation of 488 nm. Compared with the usual probes, the modified HAP nanoparticles were more photostable and were almost nontoxic. It was important that the HAP characteristics remained after the surface modification with a small amount of Tb. After incubating with rabbit bone marrow mesenchymal stem cells (MSCs) in culture, the luminescence of the internalized HAP in the living cells was clearly observed under a fluorescent microscope. Transmission electron microscopy analysis also confirmed the uptake of the particles by MSCs. It was demonstrated that the modified HAP was a stable biological probe for cellular research. We suggested that the Tb-doped HAP should have a great potential to trace the evolvement of nanoparticles, which is a key topic in nanobiotechnology.