Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis
Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis
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DOI：
10.1038/sj.emboj.7600782
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发表时间：
2005-09-07
期刊：
影响因子：
11.4
通讯作者：
Hirata, D
中科院分区：
文献类型：
--
作者：
Kanai, M;Kume, K;Hirata, D
细胞形态发生对于所有真核生物的发育、分化和细胞增殖都具有根本意义。在裂殖酵母中，果蝇 Furry-like Mor2 与 NDR/Tricornered 激酶 Orb6 协同作用，在细胞形态发生中发挥重要作用。这些基因的突变导致细胞极性丧失。在这里，我们展示了保守蛋白，MO25 样 Pmo25、GC 激酶 Nak1、Mor2 和 Orb6，构成了一个对于极性控制和细胞分离非常重要的形态发生网络。有趣的是，Pmo25 定位于有丝分裂纺锤体极体 (SPB)，然后在胞质分裂时易位至分裂内侧区域。 Pmo25 与 Nak1 形成复合物，并且是 Nak1 的定位和激酶活性所必需的。 Pmo25 和 Nak1 又对 Orb6 激酶活性至关重要。此外，SPB 上的 Pmo25 定位和分裂间期的 Nak1-Orb6 激酶活性受到分隔启动网络 (SIN) 中 Cdc7 和 Sid1 激酶的控制，表明 SIN 与胞质分裂后细胞形态发生/分离网络之间存在功能联系。
Cell morphogenesis is of fundamental significance in all eukaryotes for development, differentiation, and cell proliferation. In fission yeast, Drosophila Furry-like Mor2 plays an essential role in cell morphogenesis in concert with the NDR/Tricornered kinase Orb6. Mutations of these genes result in the loss of cell polarity. Here we show that the conserved proteins, MO25-like Pmo25, GC kinase Nak1, Mor2, and Orb6, constitute a morphogenesis network that is important for polarity control and cell separation. Intriguingly, Pmo25 was localized at the mitotic spindle pole bodies (SPBs) and then underwent translocation to the dividing medial region upon cytokinesis. Pmo25 formed a complex with Nak1 and was required for both the localization and kinase activity of Nak1. Pmo25 and Nak1 in turn were essential for Orb6 kinase activity. Further, the Pmo25 localization at the SPBs and the Nak1-Orb6 kinase activities during interphase were under the control of the Cdc7 and Sid1 kinases in the septation initiation network (SIN), suggesting a functional linkage between SIN and the network for cell morphogenesis/ separation following cytokinesis.