Kit-positive interstitial cells in the rabbit urethra: structural relationships with nerves and smooth muscle
Kit-positive interstitial cells in the rabbit urethra: structural relationships with nerves and smooth muscle
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DOI：
10.1111/j.1464-410x.2006.06617.x
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发表时间：
2007-03-01
影响因子：
4.5
通讯作者：
McCloskey, Karen D.
中科院分区：
文献类型：
--
作者：
Lyons, Alan D.;Gardiner, Tom A.;McCloskey, Karen D.
为了利用Kit受体的抗体鉴定兔尿道壁中的间质细胞（IC），并检查它们的位置、形态以及与神经和平滑肌细胞（SMC）的关系，因为对从兔尿道酶促分离的细胞的研究已经确定存在显示自发电活动并具有IC的形态学特性的特化细胞。用抗体标记并用共聚焦显微镜检查。将一些标本包埋在石蜡中并进行组织学处理。用Masson三色染色法对家兔尿道近端1/3和中段1/3区域的组织切片进行细胞排列观察，两个区域的切片均具有外纵层和内环层的SM，以及含有结缔组织和血管的固有层，管腔内排列着尿路上皮细胞。中三分之一区域具有比最近端样品更发达的圆形SM层，并且在固有层中具有广泛的内部纵向SM束。用抗试剂盒标记显示兔尿道壁内的免疫阳性细胞，在肌层的环形和纵向层中。SM肌球蛋白的抗体双标记显示，在SM束的边界上有Kit阳性细胞，其方向平行于束的轴。其他人在束之间的空间，经常互相接触。Kit阳性细胞呈细长形，有几个侧枝，或星状，有来自中央索马的分支。类似的细胞可以用波形蛋白抗体标记。它们与壁内神经的关系通过抗神经丝抗体的双重免疫染色来检查。在兔尿道平滑肌层内、平滑肌束边缘及束间间隙内均有Kit阳性细胞与神经的接触，与神经的接触部位也有类似的变化。与含nNOS的神经元接触可能意味着参与尿道的氮能抑制性神经传递。
To identify interstitial cells (ICs) in the wall of the rabbit urethra using antibodies to the Kit receptor, and to examine their location, morphology and relationship with nerves and smooth muscle cells (SMCs), as studies of enzymatically isolated cells from the rabbit urethra have established that there are specialized cells that show spontaneous electrical activity and have morphological properties of ICs.Urethral tissues from rabbits were fixed, labelled with antibodies and examined with confocal microscopy. Some specimens were embedded in paraffin wax and processed for histology. Histological sections from the most proximal third and mid-third region of rabbit urethra were stained with Masson's Trichrome to show their cellular arrangement.Sections from both regions had outer longitudinal and inner circular layers of SM, and a lamina propria containing connective tissue and blood vessels; the lumen was lined with urothelial cells. The mid-third region had a more developed circular SM layer than the most-proximal samples, and had extensive inner longitudinal SM bundles in the lamina propria. Labelling with anti-Kit revealed immunopositive cells within the wall of the rabbit urethra, in the circular and longitudinal layers of the muscularis. Double-labelling with an antibody to SM myosin showed Kit-positive cells on the boundary of the SM bundles, orientated parallel to the axis of the bundles. Others were in spaces between the bundles and often made contact with each other. Kit-positive cells were either elongated, with several lateral branches, or stellate with branches coming from a central soma. Similar cells could be labelled with vimentin antibodies. Their relationship with intramural nerves was examined by double immunostaining with an anti-neurofilament antibody. There were frequent points of contact between Kit-positive cells and nerves, with similar findings in specimens double-immunostained with anti-neuronal nitric oxide synthase (nNOS).Kit-positive ICs were found within the SM layers of the rabbit urethra, in association with nerves, on the edge of SM bundles and in the interbundle spaces. The contact with nNOS-containing neurones might imply participation in the nitrergic inhibitory neurotransmission of the urethra.