Compensation method for obtaining accurate, sub-micrometer displacement measurements of immersed specimens using electronic speckle interferometry
Compensation method for obtaining accurate, sub-micrometer displacement measurements of immersed specimens using electronic speckle interferometry
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DOI：
10.1364/boe.3.000407
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发表时间：
2012-03-01
影响因子：
3.4
通讯作者：
Downs, J. Crawford
中科院分区：
文献类型：
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作者：
Fazio, Massimo A.;Bruno, Luigi;Downs, J. Crawford
我们提出并验证了一种补偿方法，占固有的光学失真测量样品浸入水溶液中的位移。将球形橡胶试样安装在定制装置上并加压，使用电子散斑干涉法（ESPI）记录所得的表面位移。点到点的光方向计算是通过光线跟踪策略加上定制的B样条为基础的分析表示的标本形状。的补偿方法减少了由光学畸变引起的位移误差的平均幅度从35%到3%，和ESPI位移测量重复性表明，在95%的置信水平浸没试样的平均方差为16 nm。本文提出的ESPI干涉仪和数值数据分析程序提供了可靠的，准确的，和可重复的测量亚微米变形的球形试样浸泡在盐水溶液中的加压试验。该方法可用于量化生物组织样品在负载下的小变形，同时保持必要的水合作用，以确保准确的材料性能评估。(c)2012美国光学学会
We proposed and validated a compensation method that accounts for the optical distortion inherent in measuring displacements on specimens immersed in aqueous solution. A spherically-shaped rubber specimen was mounted and pressurized on a custom apparatus, with the resulting surface displacements recorded using electronic speckle pattern interferometry (ESPI). Point-to-point light direction computation is achieved by a ray-tracing strategy coupled with customized B-spline-based analytical representation of the specimen shape. The compensation method reduced the mean magnitude of the displacement error induced by the optical distortion from 35% to 3%, and ESPI displacement measurement repeatability showed a mean variance of 16 nm at the 95% confidence level for immersed specimens. The ESPI interferometer and numerical data analysis procedure presented herein provide reliable, accurate, and repeatable measurement of sub-micrometer deformations obtained from pressurization tests of spherically-shaped specimens immersed in aqueous salt solution. This method can be used to quantify small deformations in biological tissue samples under load, while maintaining the hydration necessary to ensure accurate material property assessment. (c) 2012 Optical Society of America