SINE retroposons can be used in vivo as nucleation centers for de novo methylation
SINE retroposons can be used in vivo as nucleation centers for de novo methylation
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DOI：
10.1128/mcb.20.10.3434-3441.2000
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发表时间：
2000-05-01
影响因子：
5.3
通讯作者：
Deragon, JM
中科院分区：
文献类型：
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作者：
Arnaud, P;Goubely, C;Deragon, JM
西内斯（short interspersed elements）是一类广泛存在于真核生物中的转座因子。利用基因组测序技术，我们观察到植物S1 SINE逆转座子主要整合在低甲基化DNA区域，并被甲基化酶靶向。然后甲基化可以从SME扩散到侧翼基因组序列中，产生远端表观遗传修饰。这种甲基化扩散是矢量定向的上游或下游的S1元件，这表明它可以促进当一个潜在的良好的甲基化序列是单链DNA复制过程中，特别是当位于滞后链。因此，短甲基化DNA区域的复制可能导致上游或下游相邻序列的从头甲基化。
SINEs (short interspersed elements) are an abundant class of transposable elements found in a,vide variety of eukaryotes. Using the genomic sequencing technique, we observed that plant S1 SINE retroposons mainly integrate in hypomethylated DNA regions and are targeted by methylases. Methylation can then spread from the SME into flanking genomic sequences, creating distal epigenetic modifications. This methylation spreading is vectorially directed upstream or downstream of the S1 element, suggesting that it could be facilitated when a potentially good methylatable sequence is single stranded during DNA replication, particularly when located on the lagging strand. Replication of a short methylated DNA region could thus lead to the de novo methylation of upstream or downstream adjacent sequences.