Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis.
Collagen fibril surface displays a constellation of sites capable of promoting fibril assembly, stability, and hemostasis.
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DOI：
10.3109/03008207.2010.511354
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发表时间：
2011-02
影响因子：
2.9
通讯作者：
San Antonio JD
中科院分区：
文献类型：
--
作者：
Orgel JP;Antipova O;Sagi I;Bitler A;Qiu D;Wang R;Xu Y;San Antonio JD
纤维性胶原形成器官和组织的结构基础，包括脉管系统、骨骼和肌腱。它们也是动态的、有组织的支架，为配体、细胞和血小板提供结合和识别位点。我们解释了最近发表的x射线衍射结果，并使用原子力显微镜数据来说明对胶原原纤维功能组织的新见解的意义。这些数据表明，胶原蛋白最关键的功能域主要集中在重叠区域，包括我们称之为“主控制区”的一系列位点。此外，胶原蛋白最暴露的部分包含其最稳定的部分——c端区域，它控制胶原蛋白的组装、交联和血液凝固。隐藏在纤维表面之下的是一组“隐”序列，它们在组织损伤和再生过程中促进止血和细胞-胶原相互作用。这些发现开始解决几个重要的、以前未解决的问题：功能结构域是如何在原纤维中组织的，哪些结构域是可访问的，哪些需要蛋白质水解或结构损伤才能暴露。在这里，我们推测胶原纤维组织如何影响与组织生长、发育和修复有关的分子过程。
Fibrillar collagens form the structural basis of organs and tissues including the vasculature, bone, and tendon. They are also dynamic, organizational scaffolds that present binding and recognition sites for ligands, cells, and platelets. We interpret recently published X-ray diffraction findings and use atomic force microscopy data to illustrate the significance of new insights into the functional organization of the collagen fibril. These data indicate that collagen’s most crucial functional domains localize primarily to the overlap region, comprising a constellation of sites we call the ‘master control region’. Moreover, collagens most exposed aspect contains its most stable part - the C-terminal region that controls collagen assembly, cross-linking, and blood clotting. Hidden beneath the fibril surface exists a constellation of ‘cryptic’ sequences poised to promote hemostasis and cell-collagen interactions in tissue injury and regeneration. These findings begin to address several important, and previously unresolved, questions: How functional domains are organized in the fibril, which domains are accessible, and which require proteolysis or structural trauma to become exposed. Here we speculate as to how collagen fibrillar organization impacts molecular processes relating to tissue growth, development, and repair.