Swainsonine-producing fungal endophytes from major locoweed species in China
Swainsonine-producing fungal endophytes from major locoweed species in China
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DOI：
10.1016/j.toxicon.2010.03.020
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发表时间：
2010-09-01
期刊：
影响因子：
2.8
通讯作者：
Li, Qinfan
中科院分区：
文献类型：
--
作者：
Yu, Yongtao;Zhao, Qingmei;Li, Qinfan
疯草包括有毒的黄芪属和棘豆属。广泛分布于中国西部地区，并导致一种称为动物疯疯癫癫的慢性神经系统疾病。为确定我国主要疯草中是否存在产苦马豆素的内生真菌，从8种疯草中分离得到了产苦马豆素的内生真菌。variabilis、尖吻金丝桃A. strictus、狭叶O. glacialis，冰草O. kansuensis、甘肃野螟O. ochrocepala、黄毛藓O. serrychetala，O. glabra和O.宽苞的。七种疯草中含有大量苦马豆素，并感染产生苦马豆素的内生真菌。根据真菌形态学特征和ITS序列的系统发育分析，将这些内生菌分类为棘豆弯丝菌（Undifilim oxytropis）。IGS区的PCR-RFLP分析表明，疯草内生菌的IGS区存在种内或种间变异。(C)2010爱思唯尔有限公司版权所有。
Locoweeds including the toxic species of Astragalus spp and Oxytropis spp. are widely distributed in the western region of China and result in a chronic neurological disease known as locoism in animals. To determine the presence of swainsonine-producing fungal endophyte of major locoweed species in China, endophytes were isolated from 8 locoweed species that including A. variabilis, A. strictus, O. glacialis, O. kansuensis, O. ochrocepala, O. sericopetala, O. glabra and O. latibracteata. Seven species of locoweed were confirmed contain substantial amounts of swainsonine and infect swainsonine-producing fungal endophyte. These endophytes were classified as Undifilim oxytropis according to the fungal morphology and phylogenetic analysis based on sufficient ITS sequences. PCR-RFLP analysis of IGS region showed that the interspecific or intraspecific variations were present among the endophytes from different locoweed species. (C) 2010 Elsevier Ltd. All rights reserved.