MicroRNA-10b regulates the renewal of spermatogonial stem cells through Kruppel-like factor 4
MicroRNA-10b regulates the renewal of spermatogonial stem cells through Kruppel-like factor 4
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Microrna-10b 通过 kruppel 样因子 4 调节精原干细胞的更新
DOI：
10.1002/cbf.3263
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发表时间：
2017-04-01
影响因子：
3.6
通讯作者：
Wu, Ji
中科院分区：
文献类型：
--
作者：
Li, Jiang;Liu, Xiang;Wu, Ji
MicroRNAs （miRs）在精子发生中具有重要的功能，精子发生是精子干细胞（ssc）的自我更新或分化。在这里，我们报道了miR-10b在调节小鼠ssc自我更新中的新作用。我们发现miR-10b在体外小鼠SSC中高表达，并增强SSC的增殖。与对照组相比，miR-10b的下调显著增加了SSCs的凋亡。发现kruppel样因子4是miR-10b促进SSC增殖的靶基因。这些发现进一步加深了我们对ssc自我更新和分化的认识，为男性不育的诊断、治疗和预防提供了依据。
MicroRNAs (miRs) are functionally important in spermatogenesis, which is the self-renewal or differentiation of spermatogonial stem cells (SSCs). Here, we report a novel role for miR-10b in regulating the self-renewal of mouse SSCs. We showed that miR-10b was highly expressed in mouse SSCs in vitro and enhanced SSC proliferation. Knockdown of miR-10b significantly increased the apoptosis of SSCs compared with controls. Kruppel-like factor 4 was found to be a target gene of miR-10b in the enhancement of SSC proliferation. These findings further our understanding of the self-renewal and differentiation of SSCs and provide a basis for the diagnosis, treatment, and prevention of male infertility.