Computationally determined shear on cells grown in orbiting culture dishes
Computationally determined shear on cells grown in orbiting culture dishes
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DOI：
10.1007/978-0-387-74911-2_22
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Sharp, M. Keith
中科院分区：
文献类型：
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作者：
Berson, R. Eric;Purcell, Matthew R.;Sharp, M. Keith
一个新的计算模型，使用计算流体动力学（CFD），提出了描述在圆柱形细胞培养皿中的流体行为所产生的运动所赋予的定轨摇床装置。该模型允许在培养皿的底表面的整个区域（代表培养物中细胞的生长表面）上确定壁剪切应力，这在以前对于精确的定量分析来说太复杂。两个初步的情况下，显示了完整的空间分辨率的剪切盘的底部。从模型确定的最大剪应力进行比较，现有的简化点函数，只提供最大值。此外，这种新模型结合了七个参数，而以前的技术只有四个参数，从而提高了准确性。文中还讨论了计算参数的优化问题。
A new computational model, using computational fluid dynamics (CFD), is presented that describes fluid behavior in cylindrical cell culture dishes resulting from motion imparted by an orbital shaker apparatus. This model allows for the determination of wall shear stresses over the entire area of the bottom surface of a dish (representing the growth surface for cells in culture) which was previously too complex for accurate quantitative analysis. Two preliminary cases are presented that show the complete spatial resolution of the shear on the bottom of the dishes. The maximum shear stress determined from the model is compared to an existing simplified point function that provides only the maximum value. Furthermore, this new model incorporates seven parameters versus the four in the previous technique, providing improved accuracy. Optimization of computational parameters is also discussed.